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pCAG/ATP8B1-HA

Expression vector of human ATP8B1 for mammalian cells, C-terminal HA-tag.

Catalog number RDB20131
Resource name pCAG/ATP8B1-HA
Clone info. Expression vector of human ATP8B1 for mammalian cells, C-terminal HA-tag. The plasmid requires co-transfection with RDB20145 pcDNA3/HF-CDC50A to ensure the cellular localization of the encoded protein upon overexpression. (References: Takatsu et al., JBC 2011, 286, 38159; Bryde et al., JBC 2010, 285, 40562; van der Velden et al., JBC 2010, 285, 40088)
Vector backbone pCAG (plasmid)
Gene/insert name human ATP8B1 cDNA; HA-tag.
Depositor|Developer Shin, Hye-Won |
 
Sequence (full) RDB20131hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB20131_B3Kfp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takatsu, H. et al., J. Biol. Chem. 286(44): 38159-38167, 2011) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Takatsu, H. et al., J. Biol. Chem. 286(44): 38159-38167, 2011)。非営利学術機関の学術研究に限る。
付加的提供条件:
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
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提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20131 pCAG/ATP8B1-HA DNA solution


How to cite this biological resource

Materials & Methods section:

The pCAG/ATP8B1-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20131).

Reference section:

Takatsu, H., Baba, K., Shima, T., Umino, H., Kato, U., Umeda, M., Nakayama, K., Shin, H.W, ATP9B, a P4-ATPase (a putative aminophospholipid translocase), localizes to the trans-Golgi network in a CDC50 protein-independent manner. J. Biol. Chem. 286 (44): 38159-38167 (2011). PMID 21914794. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Takatsu, H., ATP9B, a P4-ATPase (a putative aminophospholipid translocase), localizes to the trans-Golgi network in a CDC50 protein-independent manner. J. Biol. Chem. 286 (44): 38159-38167 (2011). PMID 21914794. [link to RRC of NBRP]