Expression vector of the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA.
Drawn by SnapGene® software |
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Clone info. | Expression vector of the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA. See, Yamaguchi, A. et al., Bioconjug. Chem., 27 (1): 198-206, 2016. |
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Comment | MutationLys61, Glu131, Ala306, Phe384, Glu444 (PylRS) |
Vector backbone | pCDF-1b (kanamycin-resistant derivative) (plasmid) |
Selectable markers | E. coli (Kanamycin) |
Growth remarks | Drug resistance has been replaced by the kanamycin resistance gene. |
Gene/insert name | Methanosarcina mazei PylRS, tRNA |
Depositor|Developer | Tagami, Shunsuke | Sakamoto, Kensaku | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Atsushi Y. et al., Bioconjug Chem. 27(1): 198-206, 2015) and an acknowledgment to the DEPOSITOR are requested. The same Terms and Conditions apply to for-profit use as to not-for-profit use conducted by academic institutions. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献(Atsushi Y. et al., Bioconjug Chem. 27(1): 198-206, 2015)を引用し、謝辞の表明を必要とする。営利目的利用についても、学術機関の学術利用目的利用と同じ提供条件を適用する。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB20116 | pCDF-Mm2 | DNA solution |
Materials & Methods section:
The pCDF-Mm2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20116). |
Reference section:
Yamaguchi, A., Matsuda, T., Ohtake, K., Yanagisawa, T., Yokoyama, S., Fujiwara, Y., Watanabe, T., Hohsaka, T., Sakamoto, K., Incorporation of a Doubly Functionalized Synthetic Amino Acid into Proteins for Creating Chemical and Light-Induced Conjugates. Bioconjug. Chem. 27 (1): 198-206 (2016). PMID 26625213. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Yamaguchi, A., Incorporation of a Doubly Functionalized Synthetic Amino Acid into Proteins for Creating Chemical and Light-Induced Conjugates. Bioconjug. Chem. 27 (1): 198-206 (2016). PMID 26625213. [link to RRC of NBRP] |
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