Resource data sheet
DNA Bank Top

pCDF-Mm2 (#RDB20116)

Expression vector of the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Yamaguchi, A., Bioconjug. Chem. 27 (1): 198-206 (2016). PMID 26625213. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA. See, Yamaguchi, A. et al., Bioconjug. Chem., 27 (1): 198-206, 2016.
Comment MutationLys61, Glu131, Ala306, Phe384, Glu444 (PylRS)
Vector backbone pCDF-1b (kanamycin-resistant derivative) (plasmid)
Selectable markers E. coli (Kanamycin)
Growth conditions Kanamycin 30 μg/mL, 37oC
Growth remarks Drug resistance has been replaced by the kanamycin resistance gene.
Gene/insert name Methanosarcina mazei PylRS, tRNA
Depositor|Developer Tagami, Shunsuke | Sakamoto, Kensaku |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Atsushi Y. et al., Bioconjug Chem. 27(1): 198-206, 2015) and an acknowledgment to the DEPOSITOR are requested. The same Terms and Conditions apply to for-profit use as to not-for-profit use conducted by academic institutions.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献(Atsushi Y. et al., Bioconjug Chem. 27(1): 198-206, 2015)を引用し、謝辞の表明を必要とする。営利目的利用についても、学術機関の学術利用目的利用と同じ提供条件を適用する。

Catalog # Resource name Shipping form Fee
RDB20116 pCDF-Mm2 DNA solution

How to cite this biological resource

Materials & Methods section:

The pCDF-Mm2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20116).

Reference section:

Yamaguchi, A., Matsuda, T., Ohtake, K., Yanagisawa, T., Yokoyama, S., Fujiwara, Y., Watanabe, T., Hohsaka, T., Sakamoto, K., Incorporation of a Doubly Functionalized Synthetic Amino Acid into Proteins for Creating Chemical and Light-Induced Conjugates. Bioconjug. Chem. 27 (1): 198-206 (2016). PMID 26625213. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Yamaguchi, A., Incorporation of a Doubly Functionalized Synthetic Amino Acid into Proteins for Creating Chemical and Light-Induced Conjugates. Bioconjug. Chem. 27 (1): 198-206 (2016). PMID 26625213. [PubMed] [Article] [RRC of NBRP]