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pET21a IFN-gamma T27-L33 deletion (#RDB20088)

Bacterial expression vector of human IFN gamma deletion mutant (T27–L33).

Clone info. Bacterial expression vector of human IFN gamma deletion mutant (T27–L33). For binding activity of anti-IFN gamma mAb. See, Yasamut, U. et al., Sci. Rep. 12 (1): 7608, 2022.
Vector backbone pET-21a(+) (plasmid)
Selectable markers Ampicillin
Growth conditions LB+Amp, 37oC
Gene/insert name human IFNG cDNA
Depositor|Developer Tayapiwatana, Chatchai |
 
Sequence (full) RDB20088hts01.seq checkAssembled from experimentally sequenced data.
Publication Yasamut, U., Sci. Rep. 12 (1): 7608 (2022). [link to RRC of NBRP]
Test sheet RDB20088_B3Idp1-1.pdf 
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Umpa Yasamut et al. Sci. Rep., 12(1):7608, 2022) Limitation of recipients of the BIOLOGICAL RESOURCE to not-for-profit institutions, limitation of the use to academic research purposes.
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MTAに書く使用条件 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Umpa Yasamut et al. Sci. Rep., 12(1):7608, 2022) Limitation of recipients of the BIOLOGICAL RESOURCE to not-for-profit institutions, limitation of the use to academic research purposes.

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RDB20088 pET21a IFN-gamma T27-L33 deletion DNA solution

How to cite this biological resource

Materials & Methods section:

The pET21a IFN-gamma T27-L33 deletion was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20088).

Reference section:

Yasamut, U., Wisitponchai, T., Lee, V.S., Yamabhai, M., Rangnoi, K., Thongkum, W., Chupradit, K., Tayapiwatana, C., Determination of a distinguished interferon gamma epitope recognized by monoclonal antibody relating to autoantibody associated immunodeficiency. Sci. Rep. 12 (1): 7608 (2022). PMID 35534543. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Yasamut, U., Determination of a distinguished interferon gamma epitope recognized by monoclonal antibody relating to autoantibody associated immunodeficiency. Sci. Rep. 12 (1): 7608 (2022). PMID 35534543. [link to RRC of NBRP]