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pET21a IFN-gamma T27A F29A L30A (#RDB20087)

Bacterial expression vector of human IFN gamma triple mutant (T27A, F29A, L30A).

Clone info. Bacterial expression vector of human IFN gamma triple mutant (T27A, F29A, L30A). For binding activity of anti-IFN gamma mAb. See, Yasamut, U. et al., Sci. Rep. 12 (1): 7608, 2022.
Vector backbone pET-21a(+) (plasmid)
Selectable markers Ampicillin
Growth conditions LB+Amp, 37oC
Gene/insert name human IFNG cDNA
Depositor|Developer Tayapiwatana, Chatchai |
 
Sequence (full) RDB20087hts01.seq checkAssembled from experimentally sequenced data.
Publication Yasamut, U., Sci. Rep. 12 (1): 7608 (2022). [link to RRC of NBRP]
Test sheet RDB20087_B3Idp1-1.pdf 
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Umpa Yasamut et al. Sci. Rep., 12(1):7608, 2022) Limitation of recipients of the BIOLOGICAL RESOURCE to not-for-profit institutions, limitation of the use to academic research purposes.
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提供依頼書  [依頼書の記入例 ]
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MTAに書く使用条件 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Umpa Yasamut et al. Sci. Rep., 12(1):7608, 2022) Limitation of recipients of the BIOLOGICAL RESOURCE to not-for-profit institutions, limitation of the use to academic research purposes.

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RDB20087 pET21a IFN-gamma T27A F29A L30A DNA solution

How to cite this biological resource

Materials & Methods section:

The pET21a IFN-gamma T27A F29A L30A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20087).

Reference section:

Yasamut, U., Wisitponchai, T., Lee, V.S., Yamabhai, M., Rangnoi, K., Thongkum, W., Chupradit, K., Tayapiwatana, C., Determination of a distinguished interferon gamma epitope recognized by monoclonal antibody relating to autoantibody associated immunodeficiency. Sci. Rep. 12 (1): 7608 (2022). PMID 35534543. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Yasamut, U., Determination of a distinguished interferon gamma epitope recognized by monoclonal antibody relating to autoantibody associated immunodeficiency. Sci. Rep. 12 (1): 7608 (2022). PMID 35534543. [link to RRC of NBRP]