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MFCS1-pshh-luciferace (#RDB19990)

Luciferace reporter vector under the control of Xenopus MFCS1 enhancer and shh promoter.

Alternative name MFCS1-shh promoter-luc2
Clone info. Luciferace reporter vector under the control of Xenopus MFCS1 enhancer and shh promoter.
Comment Expression was confirmed by the depositor by luciferase assay
Vector backbone pGL4.23 (plasmid)
Selectable markers Ampicillin (E. coli)
Growth conditions LB+Amp, 37oC
Gene/insert name Xenopus laevis Zrs (MFCS1) genomic DNA; Xenopus tropicalis shh enomic DNA.
Depositor|Developer Amano, Takanori |
 
Sequence (full) RDB19990hts01.seq checkAssembled from experimentally sequenced data.
Publication Tada, R., Dev. Biol. 500: 22-30 (2023). [link to RRC of NBRP]
Test sheet RDB19990_B3G7p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested. (Tada R et al., Dev Biol. S0012-1606(23)00093-3. doi: 10.1016/j.ydbio.2023.05.009. 2023)
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Tada R et al., Dev Biol. S0012-1606(23)00093-3. doi: 10.1016/j.ydbio.2023.05.009. 2023)。
付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19990 MFCS1-pshh-luciferace DNA solution

How to cite this biological resource

Materials & Methods section:

The MFCS1-pshh-luciferace was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19990).

Reference section:

Tada, R., Higashidate, T., Amano, T., Ishikawa, S., Yokoyama, C., Kobari, S., Nara, S., Ishida, K., Kawaguchi, A., Ochi, H., Ogino, H., Yakushiji-Kaminatsui, N., Sakamoto, J., Kamei, Y., Tamura, K., Yokoyama, H., The shh limb enhancer is activated in patterned limb regeneration but not in hypomorphic limb regeneration in Xenopus laevis. Dev. Biol. 500: 22-30 (2023). PMID 37247832. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Tada, R., The shh limb enhancer is activated in patterned limb regeneration but not in hypomorphic limb regeneration in Xenopus laevis. Dev. Biol. 500: 22-30 (2023). PMID 37247832. [link to RRC of NBRP]