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pCSIIbsr-PKG-hNo.7 (#RDB19906)

Lentivirus vector of cGMP biosensor, protein kinase G (PKG) #7 with blasticidine resistance gene.


Drawn by SnapGene® software
Sequence (full) RDB19906hts01.seq
Publication Hirota, K., Endocrinology 163 (3): bqac005 (2022). [link to RRC of NBRP]
Test sheet RDB19906_B3C6p1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Lentivirus vector of cGMP biosensor, protein kinase G (PKG) #7 with blasticidine resistance gene. PKG #7 is composed of cGMP-binding domain B from cGMP-dependent protein kinase IB, which binds specifically to intracellular cGMP, and of the ECFP and cp variant of Venus, cp173Venus, as the FRET donor and acceptor, respectively. See Fig.1 of Hirota, K. et al., Endocrinology, 163 (3): bqac005 (2022).
Vector backbone pCSIIbsr (plasmid)
Selectable markers Ampicillin (E. coli), BSD (mammalian cell). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name human PRKG1 cDNA; Aequorea victoria GFP (ECFP, cp173Venus).
Depositor|Developer Matsuda, Michiyuki | Hirota, Keisho |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.((Hirota, K et al. Endocrinology. 163(3): bqac005, 2022) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hirota, K et al. Endocrinology. 163(3): bqac005, 2022)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19906 pCSIIbsr-PKG-hNo.7 DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The pCSIIbsr-PKG-hNo.7 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19906).

Reference section:

Hirota, K., Hirashima, T., Horikawa, K., Yasoda, A., Matsuda, M., C-type Natriuretic Peptide-induced PKA Activation Promotes Endochondral Bone Formation in Hypertrophic Chondrocytes. Endocrinology 163 (3): bqac005 (2022). PMID 35041746. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hirota, K., C-type Natriuretic Peptide-induced PKA Activation Promotes Endochondral Bone Formation in Hypertrophic Chondrocytes. Endocrinology 163 (3): bqac005 (2022). PMID 35041746. [link to RRC of NBRP]