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LOTUS-V/pcDNA3

Vector of LOTUS-V, a fluorescent protein, for mammalian expression.

Catalog number RDB19881
Resource name LOTUS-V/pcDNA3
Clone info. Vector of LOTUS-V, a fluorescent protein, for mammalian expression. For more information, see Fig.1 of Inagaki, S. et al., Sci. Rep., 7:42398, 2017.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name Ciona intestinalis Ci-VSP; Oplophorus gracilirostris Luciferase (NanoLuc); Aequorea victoria GFP (Venus).
Reference of insert sequence LC061443 (DDBJ accession number)
Depositor|Developer Nagai, Takeharu |
Other clones in our bank
External Database
Ciona intestinalis Ci-VSP
    Oplophorus gracilirostris Luciferase (NanoLuc)
      Aequorea victoria GFP (Venus)
            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inagaki, S. et al., Sci. Rep. 7: 42398, 2017) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
            Additional terms and conditions:
            The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Inagaki, S. et al., Sci. Rep. 7: 42398, 2017)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。

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            本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB19881 LOTUS-V/pcDNA3 Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            How to cite this biological resource

            Materials & Methods section:

            The LOTUS-V/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19881).

            Reference section:

            Inagaki, S., Tsutsui, H., Suzuki, K., Agetsuma, M., Arai, Y., Jinno, Y., Bai, G., Daniels, M.J., Okamura, Y., Matsuda, T., Nagai, T., Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging. Sci. Rep. 7: 42398 (2017). PMID 28205521. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Inagaki, S., Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging. Sci. Rep. 7: 42398 (2017). PMID 28205521. [link to RRC of NBRP]

            2023.10.05

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