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LOTUS-V/pcDNA3

Vector of LOTUS-V, a fluorescent protein, for mammalian expression.

Catalog number RDB19881
Resource name LOTUS-V/pcDNA3
Clone info. Vector of LOTUS-V, a fluorescent protein, for mammalian expression. For more information, see Fig.1 of Inagaki, S. et al., Sci. Rep., 7:42398, 2017.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name Ciona intestinalis Ci-VSP; Oplophorus gracilirostris Luciferase (NanoLuc); Aequorea victoria GFP (Venus).
Reference of insert sequence LC061443 (DDBJ accession number)
Depositor|Developer Nagai, Takeharu |
 
Sequence (full) RDB19881hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19881_B3Acp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inagaki, S. et al., Sci. Rep. 7: 42398, 2017) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Inagaki, S. et al., Sci. Rep. 7: 42398, 2017)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。

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本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19881 LOTUS-V/pcDNA3 DNA solution


How to cite this biological resource

Materials & Methods section:

The LOTUS-V/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19881).

Reference section:

Inagaki, S., Tsutsui, H., Suzuki, K., Agetsuma, M., Arai, Y., Jinno, Y., Bai, G., Daniels, M.J., Okamura, Y., Matsuda, T., Nagai, T., Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging. Sci. Rep. 7: 42398 (2017). PMID 28205521. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Inagaki, S., Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging. Sci. Rep. 7: 42398 (2017). PMID 28205521. [link to RRC of NBRP]