Resource data sheet
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YeNL/pcDNA3 (#RDB19872)

Vector of yellow enhanced-nanolantern, a chemiluminescent protein, for mammalian expression.


Drawn by SnapGene® software
Sequence (full) RDB19872hts01.seq
Publication Suzuki, K., Nat. Commun. 7: 13718 (2016). [link to RRC of NBRP]
Test sheet RDB19872_B3Acp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Vector of yellow enhanced-nanolantern, a chemiluminescent protein, for mammalian expression. For more information, see Fig.1 of Suzuki, K. et al., Nat. Commun., 7: 13718, 2016.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name Oplophorus gracilirostris Luciferase (NanoLuc); Aequorea victoria GFP (Venus).
Reference of insert sequence LC128716 (DDBJ accession number)
Depositor|Developer Nagai, Takeharu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Suzuki, K. et al., Nat. Commun. 7: 13718, 2016) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Suzuki, K. et al., Nat. Commun. 7: 13718, 2016)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。

MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19872 YeNL/pcDNA3 DNA solution

How to cite this biological resource

Materials & Methods section:

The YeNL/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19872).

Reference section:

Suzuki, K., Kimura, T., Shinoda, H., Bai, G., Daniels, M.J., Arai, Y., Nakano, M., Nagai, T., Five colour variants of bright luminescent protein for real-time multicolour bioimaging. Nat. Commun. 7: 13718 (2016). PMID 27966527. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Suzuki, K., Five colour variants of bright luminescent protein for real-time multicolour bioimaging. Nat. Commun. 7: 13718 (2016). PMID 27966527. [link to RRC of NBRP]