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OeNL/pcDNA3

Vector of orange enhanced-nanolantern, a chemiluminescent protein, for mammalian expression.

Catalog number RDB19871
Resource name OeNL/pcDNA3
Clone info. Vector of orange enhanced-nanolantern, a chemiluminescent protein, for mammalian expression. For more information, see Fig.1 of Suzuki, K. et al., Nat. Commun., 7: 13718, 2016.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name Oplophorus gracilirostris Luciferase (NanoLuc); Fungia concinna RFP (mKusabira-Orange).
Reference of insert sequence LC128717 (DDBJ accession number)
Depositor|Developer Nagai, Takeharu |
 
Sequence (full) RDB19871hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19871_B3Acp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Suzuki, K. et al., Nat. Commun. 7: 13718, 2016) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering
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MTA, for use for not-for-profit academic purpose [Word]
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Approval form [Word] by Dr. Atsushi Miyawaki
Please visit Information of Request for Distribution.[link] 
Remarks
Please obtain written approval of Dr. Atsushi Miyawaki.
提供案内 (日本国内) [open/close]

提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Suzuki, K. et al., Nat. Commun. 7: 13718, 2016)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。

付加的提供条件:
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
提供同意書 (MTA, 営利目的用)[Word]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考
宮脇敦史博士の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19871 OeNL/pcDNA3 DNA solution


How to cite this biological resource

Materials & Methods section:

The OeNL/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19871).

Reference section:

Suzuki, K., Kimura, T., Shinoda, H., Bai, G., Daniels, M.J., Arai, Y., Nakano, M., Nagai, T., Five colour variants of bright luminescent protein for real-time multicolour bioimaging. Nat. Commun. 7: 13718 (2016). PMID 27966527. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Suzuki, K., Five colour variants of bright luminescent protein for real-time multicolour bioimaging. Nat. Commun. 7: 13718 (2016). PMID 27966527. [link to RRC of NBRP]