Resource data sheet
DNA Bank Top

gTEMP/pcDNA3 (#RDB19869)

Vector of gTEMP, a genetically-encoded temperature indicator, for mammalian expression.


Drawn by SnapGene® software
Sequence (full) RDB19869hts01.seq
Publication Nakano, M., PLoS One 12 (2): e0172344 (2017). [link to RRC of NBRP]
Test sheet RDB19869_B3Acp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Vector of gTEMP, a genetically-encoded temperature indicator, for mammalian expression.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name Aequorea victoria GFP (Sirius), GFP (mT-Sapphire).
Reference of insert sequence LC132716 (DDBJ accession number)
Depositor|Developer Nagai, Takeharu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakano, M. et al., PLoS One 12 (2): e0172344, 2017) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nakano, M. et al., PLoS One 12 (2): e0172344, 2017)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19869 gTEMP/pcDNA3 DNA solution

How to cite this biological resource

Materials & Methods section:

The gTEMP/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19869).

Reference section:

Nakano, M., Arai, Y., Kotera, I., Okabe, K., Kamei, Y., Nagai, T., Genetically encoded ratiometric fluorescent thermometer with wide range and rapid response. PLoS One 12 (2): e0172344 (2017). PMID 28212432. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nakano, M., Genetically encoded ratiometric fluorescent thermometer with wide range and rapid response. PLoS One 12 (2): e0172344 (2017). PMID 28212432. [link to RRC of NBRP]