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ELP-TEMP/pcDNA3.1(-) (#RDB19861)

Vector of ELP-TEMP, a genetically encoded highly-sensitive temperature indicator, for mammalian expression.


Drawn by SnapGene® software
Sequence (full) RDB19861hts01.seq
Publication Vu, C.Q., Sci. Rep. 11 (1): 16519 (2021). [link to RRC of NBRP]
Test sheet RDB19861_B3Acp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Vector of ELP-TEMP, a genetically encoded highly-sensitive temperature indicator, for mammalian expression. For more information, see Fig.1 of Vu, C.Q. et al., Sci. Rep., 11: 16519, 2021.
Vector backbone pcDNA3.1(-) (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name Aequorea victoria GFP (mTurquoise2), GFP (mVenus); synthetic linker (ELP[AV-60]).
Depositor|Developer Nagai, Takeharu |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Vu C.Q. et al., Sci. Rep. 11 (1): 16519, 2021) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Vu C.Q. et al., Sci. Rep. 11 (1): 16519, 2021)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。


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RDB19861 ELP-TEMP/pcDNA3.1(-) DNA solution

How to cite this biological resource

Materials & Methods section:

The ELP-TEMP/pcDNA3.1(-) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19861).

Reference section:

Vu, C.Q., Fukushima, S.I., Wazawa, T., Nagai, T., A highly-sensitive genetically encoded temperature indicator exploiting a temperature-responsive elastin-like polypeptide. Sci. Rep. 11 (1): 16519 (2021). PMID 34389773. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Vu, C.Q., A highly-sensitive genetically encoded temperature indicator exploiting a temperature-responsive elastin-like polypeptide. Sci. Rep. 11 (1): 16519 (2021). PMID 34389773. [link to RRC of NBRP]