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pJNC-pGLuc

Mammalian expression vector of G. princeps Gaussia luciferase, codon-optimized (GLuc).

Catalog number RDB19855
Resource name pJNC-pGLuc
Alternative name P-111
Clone info. Mammalian expression vector of G. princeps Gaussia luciferase, codon-optimized (GLuc).
Vector backbone pCMV-JNC (pcDNA3 derivative) (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name Gaussia princeps luciferase cDNA.
Depositor|Developer JNC CORPORATION, |
 
Sequence (full) RDB19855hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19855_B2Lmp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. (Inouye, S. et al., Protein Expr. Purif. 128: 93-100, 2016) RECIPIENT must contact the JNC CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
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MTA, for use for not-for-profit academic purpose [Word]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
提供案内 (日本国内) [open/close]

提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Inouye, S. et al., Protein Expr. Purif. 128: 93-100, 2016)。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び 商業活動(*)を行う場合は、JNC株式会社と別途協議を行う。(*)商用活動とは,本件リソースが製品の一部に組み込まれる場合,本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては,営利,非営利機関等の利用者制限は定めない。

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提供依頼書 [Word]
提供同意書 (MTA, 非営利学術目的用)[Word]
提供同意書 (MTA, 営利目的用)[Word]
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19855 pJNC-pGLuc DNA solution


How to cite this biological resource

Materials & Methods section:

The pJNC-pGLuc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19855).

Reference section:

Inouye, S., Suzuki, T., Protein expression of preferred human codon-optimized Gaussia luciferase genes with an artificial open-reading frame in mammalian and bacterial cells. Protein Expr. Purif. 128: 93-100 (2016). PMID 27506135. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Inouye, S., Protein expression of preferred human codon-optimized Gaussia luciferase genes with an artificial open-reading frame in mammalian and bacterial cells. Protein Expr. Purif. 128: 93-100 (2016). PMID 27506135. [link to RRC of NBRP]