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pJNC-hINS-GLuc (#RDB19845)

Mammalian expression vector of human preproinsulin (hINS) and G. princeps Gaussia luciferase (GLuc).


Drawn by SnapGene® software
Sequence (full) RDB19845hts01.seq
Publication Suzuki, T., Anal. Biochem. 415 (2): 182-189 (2011). [link to RRC of NBRP]
Test sheet RDB19845_B2Lmp1-1.pdf 
Assembled from experimentally sequenced data.
 
Alternative name P-101
Clone info. Mammalian expression vector of human preproinsulin (hINS) and G. princeps Gaussia luciferase (GLuc).
Vector backbone pCMV-JNC (pcDNA3 derivative) (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name Gaussia princeps luciferase cDNA; human INS cDNA.
Depositor|Developer JNC CORPORATION, |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. (Suzuki, T. et al., Anal. Bichem. 415 (2): 182-189, 2011) (RECIPIENT must contact the JNC CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること（Suzuki, T. et al., Anal. Bichem. 415 (2): 182-189, 2011）。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び　商業活動(*)を行う場合は、JNC株式会社と別途協議を行う。(*)商用活動とは，本件リソースが製品の一部に組み込まれる場合，本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては，営利，非営利機関等の利用者制限は定めない。


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RDB19845 pJNC-hINS-GLuc DNA solution

How to cite this biological resource

Materials & Methods section:

The pJNC-hINS-GLuc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19845).

Reference section:

Suzuki, T., Kondo, C., Kanamori, T., Inouye, S., Video rate bioluminescence imaging of secretory proteins in living cells: localization, secretory frequency, and quantification. Anal. Biochem. 415 (2): 182-189 (2011). PMID 21477579. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Suzuki, T., Video rate bioluminescence imaging of secretory proteins in living cells: localization, secretory frequency, and quantification. Anal. Biochem. 415 (2): 182-189 (2011). PMID 21477579. [link to RRC of NBRP]