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pJN-ZZ (#RDB19844)

Expression vector of ZZ domain (Synthetic IgG-binding domain from Staphylococcal Protein A).


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Inouye, S., Biochem. Biophys. Res. Commun. 376 (3): 448-453 (2008). PMID 18789309. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name P-015
Clone info. Expression vector of ZZ domain (Synthetic IgG-binding domain from Staphylococcal Protein A).
Comment Transformants grow slowly. DNA yield is low.
Vector backbone pUC-JN (pUC9 derivative) (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Growth conditions LB+Amp, 37oC
Gene/insert name Staphylococcal IgG-binding domain.
Depositor|Developer JNC CORPORATION, |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. (Inouye, S. and Sahara, Y., Biochem. Biophys. Res. Commun. 376 (3): 448-453, 2008) RECIPIENT must contact the JNC CORPORATION in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
提供案内 (日本国内) [open/close]

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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること（Inouye, S. and Sahara, Y., Biochem. Biophys. Res. Commun. 376 (3): 448-453, 2008）。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び　商業活動(*)を行う場合は、JNC株式会社と別途協議を行う。(*)商用活動とは，本件リソースが製品の一部に組み込まれる場合，本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。そのための基礎検討の使用に関しては，営利，非営利機関等の利用者制限は定めない。


Catalog # Resource name Shipping form Fee
RDB19844 pJN-ZZ DNA solution

How to cite this biological resource

Materials & Methods section:

The pJN-ZZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19844).

Reference section:

Inouye, S., Sahara, Y., Soluble protein expression in E. coli cells using IgG-binding domain of protein A as a solubilizing partner in the cold induced system. Biochem. Biophys. Res. Commun. 376 (3): 448-453 (2008). PMID 18789309. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Inouye, S., Soluble protein expression in E. coli cells using IgG-binding domain of protein A as a solubilizing partner in the cold induced system. Biochem. Biophys. Res. Commun. 376 (3): 448-453 (2008). PMID 18789309. [PubMed] [Article] [RRC of NBRP]