RDB19817 - pUC18_delLcho3510

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pUC18_delLcho3510 (#RDB19817)

A vector for gene replacement of Lcho_3510 with kanamycin-registance gene (Kmr).

Clone info.	A vector for gene replacement of Lcho_3510 with kanamycin-registance gene (Kmr).
Vector backbone	pUC18-mob (plasmid)
Selectable markers	Kanamycin
Growth conditions	LB+Kanamycin, 37oC
Gene/insert name	Leptothrix cholodnii (SP-6 strain) Lcho_3510 genomic DNA; Bacillus subtilis spoVG genomic DNA; E. coli kanamycin resistance gene genomic DNA.
Depositor\|Developer	Kunoh, Tatsuki \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. (Kunoh, T. et al. Appl. Environ. Microbiol. e0134122, 2022. doi: 10.1128/aem.01341-22.)

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. (Kunoh, T. et al. Appl. Environ. Microbiol. e0134122, 2022. doi: 10.1128/aem.01341-22.)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Kunoh, T. et al. Appl. Environ. Microbiol. e0134122, 2022. doi: 10.1128/aem.01341-22.)。

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB19817	pUC18_delLcho3510	Under QC test. Please contact us.	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pUC18_delLcho3510 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19817).

Reference section:

Kunoh, T., Yamamoto, T., Prasad, M., Ono, E., Li, X., Sugimoto, S., Iida, E., Obana, N., Takeda, M., Nomura, N., Utada, A.S., Porous Pellicle Formation of a Filamentous Bacterium, Leptothrix. Appl. Environ. Microbiol. 88 (23): e0134122 (2022). PMID 36416549. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original	Kunoh, T., Porous Pellicle Formation of a Filamentous Bacterium, Leptothrix. Appl. Environ. Microbiol. 88 (23): e0134122 (2022). PMID 36416549. [link to RRC of NBRP]
protocol	Kunoh, T., Development of a Gene Replacement Method for the Filamentous Bacterium Leptothrix cholodnii SP-6. Bio Protoc. (8): e4652 (2023). PMID 37113333.