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pAAV-eSyn-RCaMP3-WPRE (#RDB19694)

Expression vector of genetically encoded calcium indicator, RCaMP3.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Yokoyama, T., Nat. Methods 21 (5): 897-907 (2024). PMID 38514778. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. AAV Expression vector of genetically encoded calcium indicator, RCaMP3. RCaMP3 under the control of a human SynapsinI promoter with expression enhanced by WPRE (woodchuck hepatitis virus posttranscriptional regulatory element).
Vector backbone pAAV (plasmid)
Growth conditions LB+Amp, 37oC
Depositor|Developer Sakamoto, Masayuki | Yokoyama, Tatsushi |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. (Yokoyama,T. et al., Nat. Methods 21 (5): 897-907 (2024).
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Yokoyama,T. et al., Nat. Methods 21 (5): 897-907 (2024)。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee
RDB19694 pAAV-eSyn-RCaMP3-WPRE DNA solution

How to cite this biological resource

Materials & Methods section:

The pAAV-eSyn-RCaMP3-WPRE was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19694).

Reference section:

Yokoyama, T., Manita, S., Uwamori, H., Tajiri, M., Imayoshi, I., Yagishita, S., Murayama, M., Kitamura, K., Sakamoto, M., A multicolor suite for deciphering population coding of calcium and cAMP in vivo. Nat. Methods 21 (5): 897-907 (2024). PMID 38514778. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Yokoyama, T., A multicolor suite for deciphering population coding of calcium and cAMP in vivo. Nat. Methods 21 (5): 897-907 (2024). PMID 38514778. [PubMed] [Article] [RRC of NBRP]