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pAAV-eSyn-cAMPinG1-ST-WPRE (#RDB19693)

Expression vector of genetically encoded cAMP indicator, cAMPinG1 with soma targeted signal.


Drawn by SnapGene® software
Sequence (full) RDB19693hts01.seq
Publication Yokoyama, T., Nat. Methods 21 (5): 897-907 (2024). [link to RRC of NBRP]
Test sheet RDB19693_B2Lqp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. AAV vector of genetically encoded cAMP indicator, cAMPinG1 with soma targeted signal. cAMPinG1-ST under the control of a human SynapsinI promoter with expression enhanced by WPRE (woodchuck hepatitis virus posttranscriptional regulatory element).
Vector backbone pAAV (plasmid)
Depositor|Developer Sakamoto, Masayuki | Yokoyama, Tatsushi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Kyoto University MTA [Word] is also required.
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution (i) The Recipient shall enter into a separate material transfer agreement with Kyoto University prior to making a request for the Biological Resource. The Recipient shall provide RIKEN BRC with a copy of the above material transfer agreement to certify the Kyoto University's consent. (ii) The Biological Resource shall only be used by a not-for-profit organization for teaching and academic research purposes. (iii) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. (Yokoyama,T. et al., Nat. Methods 21 (5): 897-907 (2024).
Remarks Remember that you will be working with samples containing infectious virus.
Please send a partially executed MTA between the RECIPIENT and Kyoto University (Kyoto University MTA) to Dr. Masayuki Sakamoto (sakamoto.masayuki.2e[at]kyoto-u.ac.jp).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
京都大学MTA [Word]とのMTAも必要です
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 遺伝子材料の提供依頼に先立ち、利用者は京都大学と別途 MTA を締結する。利用者は京都大学と別途締結した上記MTAの写しを理研BRCに提出する。非営利機関の教育目的あるいは学術研究目的の使用にのみに提供する。利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Yokoyama,T. et al., Nat. Methods 21 (5): 897-907 (2024)。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
京都大学とのMTA(京都大学MTA)は坂本雅行博士 (sakamoto.masayuki.2e[at]kyoto-u.ac.jp)に送付してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19693 pAAV-eSyn-cAMPinG1-ST-WPRE DNA solution

How to cite this biological resource

Materials & Methods section:

The pAAV-eSyn-cAMPinG1-ST-WPRE was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19693).

Reference section:

Yokoyama T, Manita S, Uwamori H, Tajiri M, Imayoshi I, Yagishita S, Murayama M, Kitamura K, Sakamoto M, A multicolor suite for deciphering population coding of calcium and cAMP in vivo. Nat. Methods 21 (5): 897-907 (2024). PMID 38514778. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Yokoyama, T., A multicolor suite for deciphering population coding of calcium and cAMP in vivo. Nat. Methods 21 (5): 897-907 (2024). PMID 38514778. [link to RRC of NBRP]