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human VDBP/Gc1F-His (#RDB19651)

Expression vector of human vitamine D binding protein Gc1F isoform. His-tag at C-terminus.

Clone info. Expression vector of human GC vitamin D binding protein (GC), GC1F isoform, His-tag at C-terminus. See Fig.S1 and Fig.S2 of Nabeshima, Y. et al. Sci. Rep., 10 (1): 19122, 2020.
Comment Expression was confirmed by the depositow with column chromatography and Western blotting.
Vector backbone pcDNA3.4TOPO (plasmid)
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name human vitamine D binding protein (Gc1F isoform) cDNA.
Depositor|Developer Nabeshima, Yo-ichi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nabeshima, Y. et al. Sci. Rep. 10(1): 19122, 2020)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nabeshima, Y. et al. Sci. Rep. 10(1): 19122, 2020)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19651 human VDBP/Gc1F-His Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

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How to cite this biological resource

Materials & Methods section:

The human VDBP/Gc1F-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19651).

Reference section:

Nabeshima, Y., Abe, C., Kawauchi, T., Hiroi, T., Uto, Y., Nabeshima, Y.I., Simple method for large-scale production of macrophage activating factor GcMAF. Sci. Rep. 10 (1): 19122 (2020). PMID 33154460. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Nabeshima, Y., Simple method for large-scale production of macrophage activating factor GcMAF. Sci. Rep. 10 (1): 19122 (2020). PMID 33154460. [link to RRC of NBRP]