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pcDNA3.1-ALuc49-KDEL (#RDB19617)

Expression vector of artificial luciferase variant ALuc49 derived from the copepod gene database for mammalian cell imaging.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Kim, S.B., ACS Comb. Sci. 19 (9): 594-599 (2017). PMID 28742969. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name ALuc49
Clone info. Expression vector of Artificial luciferase variant-49 (ALuc49) derived from the copepod gene database for mammalian cell imaging. See Fig. 3A of Kim, S.B. et al. ACS Comb. Sci. 19 (9): 594-599, 2017.
Comment ALuc49 (GenBank: MF817976), the N-terminal end of which naturally comprises a secretion peptide (SP). To suppress the secretion nature, the C-terminal end was modified with an ER-localization signal (i.e., KDEL). This luciferase is supposed to be sequestered in the ER in mammalian cells after expression. Nevertheless, a low level of the luciferase is still secreted into the extracellular compartment.
Vector backbone pcDNA3.1(+) (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell).
Growth conditions LB+Amp, 37℃
Gene/insert name copepod Luciferase cDNA.
Reference of insert sequence MF817976 (DDBJ accession number)
Depositor|Developer Kim, Sung Bae |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of one of the literature designated by the DEPOSITOR is requested. (Sung Bae Kim et al. Bioconjug Chem. 24(12):2067-75, 2013); (Sung Bae Kim et al. ACS Comb Sci. 19(9):594-599, 2017); (Shota, T. et al. Scientific reports, 11, 2219, 2021) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定するいずれかの文献を引用する (Sung Bae Kim et al. Bioconjug Chem. 24(12):2067-75, 2013); (Sung Bae Kim et al. ACS Comb Sci. 19(9):594-599, 2017); (Shota, T. et al. Scientific reports, 11, 2219, 2021)。学術機関の学術研究に限る。



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RDB19617 pcDNA3.1-ALuc49-KDEL DNA solution Please contact us.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-ALuc49-KDEL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19617).

Reference section:

Kim, S.B., Nishihara, R., Citterio, D., Suzuki, K., Fabrication of a New Lineage of Artificial Luciferases from Natural Luciferase Pools. ACS Comb. Sci. 19 (9): 594-599 (2017). PMID 28742969. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kim, S.B., Fabrication of a New Lineage of Artificial Luciferases from Natural Luciferase Pools. ACS Comb. Sci. 19 (9): 594-599 (2017). PMID 28742969. [PubMed] [Article] [RRC of NBRP]
reference Kim, S.B., Bright Molecular Strain Probe Templates for Reporting Protein-Protein Interactions. Sensors (Basel) 23 (7): 3498 (2023). PMID 37050557. [PubMed] [Article]
reference Kim, S.B., Creation of Artificial Luciferase 60s from Sequential Insights and Their Applications to Bioassays. Sensors (Basel) 23 (14): 6376 (2023). PMID 37514669. [PubMed] [Article]
reference Kamiya, G., Multiplex quadruple bioluminescent assay system. Sci. Rep. 12 (1): 17485 (2022). PMID 36261452. [PubMed] [Article]