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ALuc23 (#RDB19616)

Expression vector of artificial luciferase variant ALuc23 derived from the copepod gene database for mammalian cell imaging.

Clone info. Expression vector of Artificial luciferase variant-23 (ALuc23) derived from the copepod gene database for mammalian cell imaging. See Fig. 1B and Fig. S2 of Kim, S.B. et al. Bioconjug. Chem. 24 (12): 2067-2075, 2013.
Comment ALuc23 (GenBank: MF817968), the N-terminal end of which naturally comprises a secretion peptide (SP). To suppress the secretion nature, the C-terminal end was modified with an ER-localization signal (i.e., KDEL). This luciferase is supposed to be sequestered in the ER in mammalian cells after expression. Nevertheless, a low level of the luciferase is still secreted into the extracellular compartment.
Vector backbone pcDNA3.1(+) (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell).
Gene/insert name copepod Luciferase cDNA.
Reference of insert sequence MF817968 (DDBJ accession number)
Depositor|Developer Kim, Sung Bae |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of one of the literature designated by the DEPOSITOR is requested. (Sung Bae Kim et al. Bioconjug Chem. 24(12):2067-75, 2013); (Sung Bae Kim et al. ACS Comb Sci. 19(9):594-599, 2017); (Shota, T. et al. Scientific reports, 11, 2219, 2021) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.
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提供依頼書  [依頼書の記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定するいずれかの文献を引用する (Sung Bae Kim et al. Bioconjug Chem. 24(12):2067-75, 2013); (Sung Bae Kim et al. ACS Comb Sci. 19(9):594-599, 2017); (Shota, T. et al. Scientific reports, 11, 2219, 2021)。学術機関の学術研究に限る。

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RDB19616 ALuc23 Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The ALuc23 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19616).

Reference section:

Kim, S.B., Torimura, M., Tao, H., Creation of artificial luciferases for bioassays. Bioconjug. Chem. 24 (12): 2067-2075 (2013). PMID 24237362. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Kim, S.B., Creation of artificial luciferases for bioassays. Bioconjug. Chem. 24 (12): 2067-2075 (2013). PMID 24237362. [link to RRC of NBRP]
reference Kamiya, G., S-Series Coelenterazine-Driven Combinatorial Bioluminescence Imaging Systems for Mammalian Cells. Int. J. Mol. Sci. 24 (2): 1420 (2023). PMID 36674934.
reference Kim, S.B., Bright Molecular Strain Probe Templates for Reporting Protein-Protein Interactions. Sensors (Basel) 23 (7): 3498 (2023). PMID 37050557.
reference Tamaki, S., Color-tunable bioluminescence imaging portfolio for cell imaging. Sci. Rep. 11 (1): 2219 (2021). PMID 33500496.
review Kim, S.B., Ligand-activated BRET9 imaging for measuring protein-protein interactions in living mice. Chem. Commun. (Camb) 56 (2): 281-284 (2019). PMID 31807738.