Resource data sheet
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pCAG-mCherry (#RDB19604)

Expression vector of red fluorescent protein, mCherry.


Drawn by SnapGene® software
Sequence (full) RDB19604hts02.seq
Publication Sakamoto, M., STAR Protoc. 3 (2): 101421 (2022). PMID 35693213. [link to RRC of NBRP]
Test sheet RDB19604_B3Kkp1-2.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Expression vector of red fluorescent protein, mCherry, SV40-polyA signal, CAG promoter.
Vector backbone pCAGpro-C1 (plasmid)
Selectable markers Kanamycin (E. coli), Neomycin (mammaliancell)
Growth conditions LB+Kan, 37oC
Gene/insert name Discosoma sp. RFP (mCherry)
Depositor|Developer Bito, Haruhiko | Sakamoto, Masayuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Sakamoto, M., STAR Protoc. 3 (2): 101421 (2022)) designated by the DEPOSITOR is requested.
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Sakamoto, M., STAR Protoc. 3 (2): 101421 (2022)) を引用する。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19604 pCAG-mCherry DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pCAG-mCherry was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19604).

Reference section:

Sakamoto, M., Ota, K., Kondo, Y., Okamura, M., Fujii, H., Bito, H., In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice. STAR Protoc. 3 (2): 101421 (2022). PMID 35693213. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Sakamoto, M., In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice. STAR Protoc. 3 (2): 101421 (2022). PMID 35693213. [link to RRC of NBRP]