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CSII-CA-MCS-IRES-Hyg (#RDB19593)

Backbone vector plasmid of lentivirus construct driven by CAG promoter with hygromycin resistance marker.

Clone info. Backbone vector plasmid of lentivirus construct driven by CAG promoter with hygromycin resistance marker.
Comment Made from CSII-EF-MCS（RDB04378) by replacing EF-1alpha promoter with CAG promoter derived from RDB07099. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSII-EF-MCS (RDB04378) (Plasmid)
Selectable markers Ampicillin (E. coli), Hygromycin (mammalian cell)
Growth conditions LB+Amp, 30oC; Use NEBstable or equivalent to amplify this clone.
Growth remarks Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r. NEB Stable or an equivalent host is recommended for amplifying this clone.
Depositor|Developer Miyazono, Kohei |
 
Sequence (full) RDB19593hts01.seq checkAssembled from experimentally sequenced data.
Publication Ozawa, T., iScience 24 (5): 102488 (2021). PMID 34113826. [link to RRC of NBRP]
Test sheet RDB19593B0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ozawa, T. et al., iScience. 24(5): 102488, 2021)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ozawa, T. et al., iScience. 24(5): 102488, 2021)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19593 CSII-CA-MCS-IRES-Hyg DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CSII-CA-MCS-IRES-Hyg was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19593).

Reference section:

Ozawa, T., Morikawa, M., Morishita, Y., Ogikubo, K., Itoh, F., Koinuma, D., Nygren, P.Å., Miyazono, K., Systemic administration of monovalent follistatin-like 3-Fc-fusion protein increases muscle mass in mice. iScience 24 (5): 102488 (2021). PMID 34113826. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ozawa, T., Systemic administration of monovalent follistatin-like 3-Fc-fusion protein increases muscle mass in mice. iScience 24 (5): 102488 (2021). PMID 34113826. [link to RRC of NBRP]