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CSII-CA-MCS-IRES-Puro

Backbone vector plasmid of lentivirus construct driven by CAG promoter with puromycin resistance marker.

Catalog number RDB19592
Resource name CSII-CA-MCS-IRES-Puro
Clone info. Backbone vector plasmid of lentivirus construct driven by CAG promoter with puromycin resistance marker.
Comment Made from CSII-EF-MCS（RDB04378) by replacing EF-1alpha promoter with CAG promoter derived from RDB07099. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSII-EF-MCS （RDB04378) (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Growth remarks Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r. NEB Stable or an equivalent host is recommended for amplifying this clone.
Depositor|Developer Miyazono, Kohei |
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External Database
            Reference sequence
              
             
            Sequence (full) RDB19592hts01.seqcheck assembled from experimentally sequenced data
            Test sheet RDB19592_B2D5p1-1.pdf 

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ozawa, T. et al., iScience. 24(5): 102488, 2021)
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ozawa, T. et al., iScience. 24(5): 102488, 2021)。
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            RDB19592 CSII-CA-MCS-IRES-Puro DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]

            How to cite this biological resource

            Materials & Methods section:

            The CSII-CA-MCS-IRES-Puro was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19592).

            Reference section:

            Ozawa, T., Morikawa, M., Morishita, Y., Ogikubo, K., Itoh, F., Koinuma, D., Nygren, P.Å., Miyazono, K., Systemic administration of monovalent follistatin-like 3-Fc-fusion protein increases muscle mass in mice. iScience 24 (5): 102488 (2021). PMID 34113826. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            Featured content Lentiviral plasmid list (English text)
            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            References

            Original, user report and related articles

            original Ozawa, T., Systemic administration of monovalent follistatin-like 3-Fc-fusion protein increases muscle mass in mice. iScience 24 (5): 102488 (2021). PMID 34113826. [link to RRC of NBRP]

            2023.09.08

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