Resource data sheet
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CSII-CA-MCS-IRES-Puro (#RDB19592)

Backbone vector plasmid of lentivirus construct driven by CAG promoter with puromycin resistance marker.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Ozawa, T., iScience 24 (5): 102488 (2021). PMID 34113826. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Backbone vector plasmid of lentivirus construct driven by CAG promoter with puromycin resistance marker.
Comment Made from CSII-EF-MCS（RDB04378) by replacing EF-1alpha promoter with CAG promoter derived from RDB07099. pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) are popular choices for lentivirus packaging.
Vector backbone CSII-EF-MCS (RDB04378) (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Growth conditions LB+Amp, 30℃
Growth remarks Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r. NEB Stable or an equivalent host is recommended for amplifying this clone.
Depositor|Developer Miyazono, Kohei |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ozawa, T. et al., iScience. 24(5): 102488, 2021)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ozawa, T. et al., iScience. 24(5): 102488, 2021)。

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RDB19592 CSII-CA-MCS-IRES-Puro DNA solution

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CSII-CA-MCS-IRES-Puro was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19592).

Reference section:

Ozawa, T., Morikawa, M., Morishita, Y., Ogikubo, K., Itoh, F., Koinuma, D., Nygren, P.Å., Miyazono, K., Systemic administration of monovalent follistatin-like 3-Fc-fusion protein increases muscle mass in mice. iScience 24 (5): 102488 (2021). PMID 34113826. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ozawa, T., Systemic administration of monovalent follistatin-like 3-Fc-fusion protein increases muscle mass in mice. iScience 24 (5): 102488 (2021). PMID 34113826. [PubMed] [Article] [RRC of NBRP]