Resource data sheet
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Alcadein beta-FLAG /pcDNA3.1(+) (#RDB19588)

Expression vector of human CLSTN3 tagged with FLAG at C-terminus.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Hata, S., J. Biol. Chem. 284 (52): 36024-36033 (2009). PMID 19864413. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of human Alcadein beta/Calsyntenin-3 tagged with FLAG at C-terminus. See Fig.1 of SOBU, Y. et al., Mol. Biol. Cell. 28 (26), 3844-3856, 2017.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pcDNA3.1(+) (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cells)
Growth conditions LB+Amp, 37oC
Gene/insert name human CLSTN3 cDNA.
Depositor|Developer Suzuki, Toshiharu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Saori, H. et al., J. Biol. Chem. 284(52): 36024-36033, 2009)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Saori, H. et al., J. Biol. Chem. 284(52): 36024-36033, 2009)。

Catalog # Resource name Shipping form Fee
RDB19588 Alcadein beta-FLAG /pcDNA3.1(+) DNA solution

How to cite this biological resource

Materials & Methods section:

The Alcadein beta-FLAG /pcDNA3.1(+) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19588).

Reference section:

Hata, S., Fujishige, S., Araki, Y., Kato, N., Araseki, M., Nishimura, M., Hartmann, D., Saftig, P., Fahrenholz, F., Taniguchi, M., Urakami, K., Akatsu, H., Martins, R.N., Yamamoto, K., Maeda, M., Yamamoto, T., Nakaya, T., Gandy, S., Suzuki, T., Alcadein cleavages by amyloid beta-precursor protein (APP) alpha- and gamma-secretases generate small peptides, p3-Alcs, indicating Alzheimer disease-related gamma-secretase dysfunction. J. Biol. Chem. 284 (52): 36024-36033 (2009). PMID 19864413. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hata, S., Alcadein cleavages by amyloid beta-precursor protein (APP) alpha- and gamma-secretases generate small peptides, p3-Alcs, indicating Alzheimer disease-related gamma-secretase dysfunction. J. Biol. Chem. 284 (52): 36024-36033 (2009). PMID 19864413. [PubMed] [Article] [RRC of NBRP]