Bacterial expression vector of bovine mature adrenodoxin reductase.
Clone info. | Bacterial expression vector of bovine mature adrenodoxin reductase, 97 to 1489 nt of pBAR16 (Fig.1 of Sagara. Y. et al., J. Biochem. 102 (6): 1333-1336 (1987)), without tag. tac promoter, IPTG-inducible. See, Sagara, Y. et al. Biol. Pharm. Bull., 16 (7): 627-630, 1993. |
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Comment | Expression was confirmed by the depositor with SDS-PAGE and western blotting. |
Vector backbone | pCWori+ (plasmid) |
Selectable markers | Ampicillin |
Growth remarks | AdR expression requires incubation at 30 degrees C for 24 hours after addition of IPTG. |
Gene/insert name | bovine adrenodoxin reductase cDNA |
Depositor|Developer | Sagara, Yasuhiro | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sagara, Y. et al., Biol. Pharm. Bull., 16 (7): 627-630, 1993) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sagara, Y. et al., Biol. Pharm. Bull., 16 (7): 627-630, 1993)。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB19561 | pBAR1607 | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The pBAR1607 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19561). |
Reference section:
Sagara, Y., Wada, A., Takata, Y., Waterman, M.R., Sekimizu, K., Horiuchi, T., Direct expression of adrenodoxin reductase in Escherichia coli and the functional characterization. Biol. Pharm. Bull. 16 (7): 627-630 (1993). PMID 8401393. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Sagara, Y., Direct expression of adrenodoxin reductase in Escherichia coli and the functional characterization. Biol. Pharm. Bull. 16 (7): 627-630 (1993). PMID 8401393. [link to RRC of NBRP] |
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