Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII-CMV-TMPRSS2-IRES2-Bsd (#RDB19558)

Lentivirus expression vector of human TMPRSS2, CMV promoter.

Clone info. Lentivirus expression vector of human transmembrane serine protease 2 (TMPRSS2), CMV promoter.
Comment Expression was confirmed by the depositor with immunoblotting
Vector backbone CSII-CMV-MCS-IRES2-Bsd (RDB04385) (plasmid)
Size of vector backbone 9.5 kb
Selectable markers Ampicillin (E. coli), Bsd (lentivirus transfection). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth remarks Use NEBstable or equivalent to amplify this clone. 30 degC is fine. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name human TMPRSS2 cDNA
Depositor|Developer Sasaki, Michihito |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sasaki, M. et al., PLoS Pathog., 17 (1): e1009233, 2021)
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sasaki, M. et al., PLoS Pathog., 17 (1): e1009233, 2021)。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19558 CSII-CMV-TMPRSS2-IRES2-Bsd DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII-CMV-TMPRSS2-IRES2-Bsd was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19558).

Reference section:

Sasaki, M., Uemura, K., Sato, A., Toba, S., Sanaki, T., Maenaka, K., Hall, W.W., Orba, Y., Sawa, H., SARS-CoV-2 variants with mutations at the S1/S2 cleavage site are generated in vitro during propagation in TMPRSS2-deficient cells. PLoS Pathog. 17 (1): e1009233 (2021). PMID 33476327. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19558_B2C2p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: cPPT+CTS_F (Pr0161)
Region: CMV en.2,CMVpro.2,insert 5'
Sequence file: RDB19558_B2C2a.seq check
>19558_19558_1_cPPT+CTS_F_G09_21_ABI24.ab1
    1 NNNNNNNNNN NTCNNNNNNN GTAATNNATT ACGGGGTCAT TAGTTCNTAG CCCATATATG
   61 GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC
  121 CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT
  181 TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT
  241 CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT
  301 GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC
  361 GCTATTACCA TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA GCGGTTTGAC
  421 TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA
  481 AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC CATTGACGCA AATGGGCGGT
  541 AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTGGTT TAGTGAACCG TCAGATCCGC
  601 TAGCGCTACC GGTCTCGAGA CCATGGCTTT GAACTCAGGG TCACCACCAG CTATTGGACC
  661 TTACTATGAA AACCATGGAT ACCAACCGGA AAACCCCTAT CCCGCACAGC CCACTGTGGT
  721 CCCCACTGTC TACGANGTGC ATCCGGCTCA GTACTACCCG TCCCCCGTGC CCCAGTACGC
  781 CCCGANGGTC CTGANGCANG CTTCCNACCC CGTCGTCTGC ACGCAGCCCN AATCCCCATC
  841 CGGGNNAGTG TGCACCTCNA AGANTAANAA AGCACTGTGC ATCACCTTGA NCCTGGGGAN
  901 CTTCCTCGNG GGANCTGNGC TGGNCGCTNG NCTANTCNGG AANTTCATGG GCAGCAAGNG
  961 NTCCNACTCN GGNNNANANT GNNANTNCNC NNNNANNNGC NTNNNCCCNN CTNNCTGGNG
1021 NNANNNNNNN NNN
//
Primer: IRES-R (Pr0037)
Region: IRES2,FLAG,insert 3'
Sequence file: RDB19558_B2C2b.seq check
>19558_19558_1_IRES-R_F09_18_ABI24.ab1
    1 NNNNNNNNNN GNNNNNNNNN NANNGTTAGG GGGGGGGGNG GGNNAGGGGC GGATCCGCGG
   61 CCGCTTACTT GTCGTCATCG TCTTTGTAGT CGCCGTCTGC CCTCATTTGT CGATAAATCC
  121 AGTCCGTGAA TACCATCACA TTCCCGTACA CTCCTGGTCT GTAAGCTTTG GCACAGCCAG
  181 AACCCCAGCT TGTATCCCCT ATCAGCCACC AGATATTGTT CTTCGAAGTG ACCAGAGGCC
  241 CTCCACTGTC ACCCTGGCAA GAATCGACGT TCCCCTGCAG GAAGCCGGCA CAGATCATGG
  301 CTGGTGTGAT CAGGTTGTCA TAGACATATC TGCTGTTGCA TCTCTGTGTC TCAATGAGAA
  361 GCACCTTGGC AGCGTTCAGC ACTTCTGAGG TCTTCCCTTT CTCCTCGGTG GCCCCCCACC
  421 CGGAAATCCA GCAGAGCTGT TCTGGCTGCA GCATCATGCC TGGGTTGGGC AGACACACTG
  481 GTTTCACTAG GTCGTTGAAA GTCAGAGGCT TCTGCAGCTT CATCAGCGCA ATGTCATTGT
  541 TCTTGGTCTT GGAGTCATAA TTTGGATGAG AAATCACTTT TTCTACTTGG TATCCGGCTC
  601 CATAGAACAT GAAAGATTGT CTCAAAATCC CCGCAAATGC CGTCCAATGC CATGGATTGT
  661 TAAGAGGTTT TTCCACGCAG TGGGCGGCTG TCACGATCCA CTCGGGGGTG ATGATGGAGC
  721 CTCCGCACAC GTGGACGTTC TGGACGTGCA GGCTGANCTG CCAGGGCCAG GCCCCCGGGA
  781 NCGCGCTCTC GCCGCCCACG ATCCTGCTCT GGCGGCTTGA GTTCAAGTTG ACCCCGCANG
  841 CTATACAGCG TAAAGAAACC ACTGCTTTTG AAGAACAGGC ATCACTGTGG TACAGTTTTT
  901 TATAGATATC NACATTGNCN GNACTTGNGN NCAGTTTCAT NAANCTGGTG GNNCCGCNGN
  961 CNTCCNCTAN TNNTNNGNTA NAGTAAAAAN TANNNNNANA GCCNNNGNCC NNNNNNNNNN
//
Primer: IRES-F (Pr0036)
Region: IRES2,BSD,PRE
Sequence file: RDB19558_B2C2c.seq check
>D08711A4_B2C2_1_IRES-F_E12_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNTTNNG TCGAGGTTAN NAAACGTCTA GGCCCCCCGA ACCNCGGGGA
   61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCATGGCC AAGCCTTTGT
  121 CTCAAGAAGA ATCCACCCTC ATTGAAAGAG CAACGGCTAC AATCAACAGC ATCCCCATCT
  181 CTGAAGACTA CAGCGTCGCC AGCGCAGCTC TCTCTAGCGA CGGCCGCATC TTCACTGGTG
  241 TCAATGTATA TCATTTTACT GGGGGACCTT GTGCAGAACT CGTGGTGCTG GGCACTGCTG
  301 CTGCTGCGGC AGCTGGCAAC CTGACTTGTA TCGTCGCGAT CGGAAATGAG AACAGGGGCA
  361 TCTTGAGCCC CTGCGGACGG TGCCGACAGG TGCTTCTCGA TCTGCATCCT GGGATCAAAG
  421 CCATAGTGAA GGACAGTGAT GGACAGCCGA CGGCAGTTGG GATTCGTGAA TTGCTGCCCT
  481 CTGGTTATGT GTGGGAGGGC TAAGTCGAGC CTCTAGAGTT AACATCGAGG GATCAAGCTT
  541 ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT
  601 GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT
  661 TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG
  721 GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC
  781 CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT NCGGGACTTT CGCTTTCCCC
  841 CTCCCTATTG NCACGGCGGA ACTCATCGCC GCCTGCCTTG NCCGCTGCTN GNNAGGGGCT
  901 CGNNTGNTGG GNNCTGANNN TTNCGNGGNG NTGNCGGGGA ANCTGANGNC CTTTCCATGG
  961 CTGNTCNNCN GNGNNNNCNN NNNNNNTCTG NNCGGGNNGT NNNNNTGNNN NGTCCCNTNN
1021 NNNCNNNNNT NNNNCNNNNN NTNNNNNCNN NNNNNGNNGN NNNNNNNNN
//
Primer: HIV-B_R (Pr0673)
Region: HIV-1 psi,5'LTR,CMV pro.1,CMV en.1
Sequence file: RDB19558_B2C2d.seq check
>D08711A4_B2C2_1_HIV-B_R_C12_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTTTTGGN GTACTCNCCA GTCGCCGCCC CTCGCCTCTT
   61 GCCGTGCGCG CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC
  121 GCTTTCAAGT CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC
  181 TGAGGGATCT CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC
  241 AAGGCAAGCT TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG
  301 CTCAGATCTG GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC
  361 TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG CGGAGTTGTT ACGACATTTT
  421 GGAAAGTCCC GTTGATTTTG GTGCCAAAAC AAACTCCCAT TGACGTCAAT GGGGTGGAGA
  481 CTTGGAAATC CCCGTGAGTC AAACCGCTAT CCACGCCCAT TGATGTACTG CCAAAACCGC
  541 ATCACCATGG TAATAGCGAT GACTAATACG TAGATGTACT GCCAAGTAGG AAAGTCCCAT
  601 AAGGTCATGT ACTGGGCATA ATGCCAGGCG GGCCATTTAC CGTCATTGAC GTCAATAGGG
  661 GGCGTACTTG GCATATGATA CACTTGATGT ACTGCCAAGT GGGCAGTTTA CCGTAAATAC
  721 TCCACCCATT GACGTCAATG GAAAGTCCCT ATTGNCGTTA CTATGGGAAC ATACGTCATT
  781 ATTGACGTCN ATGGGCGGGG GTCGTTGGGC GGNCAGCCAG GCGGGCCATT TANCGTANGT
  841 TATGTAACGC GGAACTNCNT ATATGGGCTA TGAACTNATG ANCCCGTAAT TGANNACTAT
  901 TAATAACNAG TCNNTNANCN NTGTCNNCGC GNATNTCTGG NCNNTACNTC NNNAANNANC
961 NNAAAANGNN TNNNCNNNAN NNNANNNNNC NNGNNNNGNC NGNNNNNNNN NN
//
Primer: bGH_rev3 (Pr0885)
Region: bGH pA,3'LTR
Sequence file: RDB19558_B2C2e.seq check
>D08711A4_B2C2_1_bGH_rev3_G12_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGG NGGNNGGGNA NACNACAGAT GGCTGGCAAC TAGAAGGCAC
   61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA
  121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA
  181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT
  241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG
  301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT
  361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT
  421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT
  481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT
  541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC
  601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG
  661 GTACCTGANG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC
  721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCNTGTTTT TCTAGGTCTC
  781 GACGGTATCG ATGCGGGGAG GCGGNCCAAA GGGANNNCCG ANTCGTCTGA NGGCNNANNN
  841 NAANANGCGG AANANGNCNC ANANCCNGNA GCNNNCNCGG GNNNGAANGN CCGCTGNNNT
  901 GANGGNCNAN NGNNNGTANC ANANNNNNNN CCNCGCANAA NCCNNNNGGN ANCNCNNNNA
961 NCANNNNTNN NANNNNNNNC NGCTTNCCCN NNNNNCCNNN NNNNNNN
//
Primer: RRE_F1 (Pr0796)
Region: RRE,cPPT_CTS
Sequence file: RDB19558_B2C2f.seq check
>D08711A4_B2C2_1_RRE_F1_D12_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN ANATTTGCTG ANGGCTATTG AGGCGCAACA
   61 GCATCTGTTG CAACTCACAG TCTGGGGCAT CAAGCAGCTC CAGGCAAGAA TCCTGGCTGT
  121 GGAAAGATAC CTAAAGGATC AACAGCTCCT GGGGATTTGG GGTTGCTCTG GAAAACTCAT
  181 TTGCACCACT GCTGTGCCTT GGAATGCTAG TTGGAGTAAT AAATCTCTGG AACAGATTTG
  241 GAATCACACG ACCTGGATGG AGTGGGACAG AGAAATTAAC AATTACACAA GCTTAATACA
  301 CTCCTTAATT GAAGAATCGC AAAACCAGCA AGAAAAGAAT GAACAAGAAT TATTGGAATT
  361 AGATAAATGG GCAAGTTTGT GGAATTGGTT TAACATAACA AATTGGCTGT GGTATATAAA
  421 ATTATTCATA ATGATAGTAG GAGGCTTGGT AGGTTTAAGA ATAGTTTTTG CTGTACTTTC
  481 TATAGTGAAT AGAGTTAGGC AGGGATATTC ACCATTATCG TTTCAGACCC ACCTCCCAAC
  541 CCCGAGGGGA CCCGACAGGC CCGAAGGAAT AGAAGAAGAA GGTGGAGAGA GAGACAGAGA
  601 CAGATCCATT CGATTAGTGA ACGGATCTAC AAATGGCAGT ATTCATCCAC AATTTTAAAA
  661 GAAAAGGGGG GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG
  721 ACATACAAAC TAAAGAATTA CNAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT
  781 ACAGGGACAG CAGAAATTCA CTTTGAATTA ATTCGATTAA TAGTAATCAA TTACGGGGTC
  841 ATNNNTTCAT AGCCCATATA TGGAGTTCCG CGTTNCATAA CTTACNGNNA ATGGCCCGCC
  901 NNGCTGANCN NCCNACGACC CCNGCCCATN GANGNCAATN ATGACGTANG TNNCCNTAGT
  961 ANCGCCNANN NGGGNNTTTN CATTGACNNC NANGGNNGGN NTATTTNNNN NNAANNNGNC
1021 NNNNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,EM7 pro,Zeo
Sequence file: RDB19558_B2C2g.seq check
>D08711A4_B2C2_1_SV40pro_ori_F_A02_02_ABI24.ab1
    1 NNNNNNNNNN NGNNNNNGNC GCCTCTGCCT CTGANCTATT CCNGAAGTAG TGAGGAGGCT
   61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT
  121 CTGATCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA
  181 CAAGGTGAGG AACTAAACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG
  241 CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT
  301 GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA
  361 GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT
  421 GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT
  481 GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA
  541 CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC
  601 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT
  661 CCTCCAGCGC GGGGNTCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC
  721 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
  781 ACTGCATTCT AGTTGTGGNT TGNCCAAACT CNTCNATGNA TCTTATCATG TCTGTNTNNC
  841 GTCNANCTCT ANCTAGANCT NNNNNAATCA TGGNCATAGC TGNTTCCTGN GTGAAATTGN
  901 TATCCNCTCN CNNTTCCNCN CNNNATACNA NCNNNNNNCA TAAAGNGNAA AGNCNGGGGN
  961 NGNCNNNNGN NNGNNCNNNC TNNNNNNNNN TNNNNNNNNG CTNNNNNNCN GCNTNNNNNN
1021 NNGNNANNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sasaki, M., SARS-CoV-2 variants with mutations at the S1/S2 cleavage site are generated in vitro during propagation in TMPRSS2-deficient cells. PLoS Pathog. 17 (1): e1009233 (2021). PMID 33476327. [link to RRC of NBRP]
reference Uemura, K., 2-thiouridine is a broad-spectrum antiviral nucleoside analogue against positive-strand RNA viruses. Proc. Natl. Acad. Sci. U.S.A. 120 (42): e2304139120 (2023). PMID 37831739.