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CSII-CMV-TMPRSS2-IRES2-Bsd

Lentivirus expression vector of human TMPRSS2, CMV promoter.

Catalog number RDB19558
Resource name CSII-CMV-TMPRSS2-IRES2-Bsd
Clone info. Lentivirus expression vector of human transmembrane serine protease 2 (TMPRSS2), CMV promoter.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. Expression was confirmed by the depositor with immunoblotting
Vector backbone CSII-CMV-MCS-IRES2-Bsd (plasmid)
Size of vector backbone 9.5 kb
Selectable markers Ampicillin (E. coli), Bsd (mammalian cell)
Growth remarks Use NEBstable or equivalent to amplify this clone. 30 degC is fine. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name human TMPRSS2 cDNA
Depositor|Developer Sasaki, Michihito |
Other clones in our bank

External Database
human TMPRSS2

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sasaki, M. et al., PLoS Pathog., 17 (1): e1009233, 2021)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sasaki, M. et al., PLoS Pathog., 17 (1): e1009233, 2021)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB19558 CSII-CMV-TMPRSS2-IRES2-Bsd DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Sasaki, M., SARS-CoV-2 variants with mutations at the S1/S2 cleavage site are generated in vitro during propagation in TMPRSS2-deficient cells. PLoS Pathog. 17 (1): e1009233 (2021). PMID 33476327.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19558_B2C2p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: cPPT+CTS_F
          Region: CMV en.2,CMVpro.2,insert 5'
          Sequence file: RDB19558_B2C2a.seq check
          >19558_19558_1_cPPT+CTS_F_G09_21_ABI24.ab1
              1 NNNNNNNNNN NTCNNNNNNN GTAATNNATT ACGGGGTCAT TAGTTCNTAG CCCATATATG
             61 GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC
            121 CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT
            181 TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT
            241 CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT
            301 GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC
            361 GCTATTACCA TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA GCGGTTTGAC
            421 TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA
            481 AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC CATTGACGCA AATGGGCGGT
            541 AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTGGTT TAGTGAACCG TCAGATCCGC
            601 TAGCGCTACC GGTCTCGAGA CCATGGCTTT GAACTCAGGG TCACCACCAG CTATTGGACC
            661 TTACTATGAA AACCATGGAT ACCAACCGGA AAACCCCTAT CCCGCACAGC CCACTGTGGT
            721 CCCCACTGTC TACGANGTGC ATCCGGCTCA GTACTACCCG TCCCCCGTGC CCCAGTACGC
            781 CCCGANGGTC CTGANGCANG CTTCCNACCC CGTCGTCTGC ACGCAGCCCN AATCCCCATC
            841 CGGGNNAGTG TGCACCTCNA AGANTAANAA AGCACTGTGC ATCACCTTGA NCCTGGGGAN
            901 CTTCCTCGNG GGANCTGNGC TGGNCGCTNG NCTANTCNGG AANTTCATGG GCAGCAAGNG
            961 NTCCNACTCN GGNNNANANT GNNANTNCNC NNNNANNNGC NTNNNCCCNN CTNNCTGGNG
          1021 NNANNNNNNN NNN
          //
          Primer: IRES-R
          Region: IRES2,FLAG,insert 3'
          Sequence file: RDB19558_B2C2b.seq check
          >19558_19558_1_IRES-R_F09_18_ABI24.ab1
              1 NNNNNNNNNN GNNNNNNNNN NANNGTTAGG GGGGGGGGNG GGNNAGGGGC GGATCCGCGG
             61 CCGCTTACTT GTCGTCATCG TCTTTGTAGT CGCCGTCTGC CCTCATTTGT CGATAAATCC
            121 AGTCCGTGAA TACCATCACA TTCCCGTACA CTCCTGGTCT GTAAGCTTTG GCACAGCCAG
            181 AACCCCAGCT TGTATCCCCT ATCAGCCACC AGATATTGTT CTTCGAAGTG ACCAGAGGCC
            241 CTCCACTGTC ACCCTGGCAA GAATCGACGT TCCCCTGCAG GAAGCCGGCA CAGATCATGG
            301 CTGGTGTGAT CAGGTTGTCA TAGACATATC TGCTGTTGCA TCTCTGTGTC TCAATGAGAA
            361 GCACCTTGGC AGCGTTCAGC ACTTCTGAGG TCTTCCCTTT CTCCTCGGTG GCCCCCCACC
            421 CGGAAATCCA GCAGAGCTGT TCTGGCTGCA GCATCATGCC TGGGTTGGGC AGACACACTG
            481 GTTTCACTAG GTCGTTGAAA GTCAGAGGCT TCTGCAGCTT CATCAGCGCA ATGTCATTGT
            541 TCTTGGTCTT GGAGTCATAA TTTGGATGAG AAATCACTTT TTCTACTTGG TATCCGGCTC
            601 CATAGAACAT GAAAGATTGT CTCAAAATCC CCGCAAATGC CGTCCAATGC CATGGATTGT
            661 TAAGAGGTTT TTCCACGCAG TGGGCGGCTG TCACGATCCA CTCGGGGGTG ATGATGGAGC
            721 CTCCGCACAC GTGGACGTTC TGGACGTGCA GGCTGANCTG CCAGGGCCAG GCCCCCGGGA
            781 NCGCGCTCTC GCCGCCCACG ATCCTGCTCT GGCGGCTTGA GTTCAAGTTG ACCCCGCANG
            841 CTATACAGCG TAAAGAAACC ACTGCTTTTG AAGAACAGGC ATCACTGTGG TACAGTTTTT
            901 TATAGATATC NACATTGNCN GNACTTGNGN NCAGTTTCAT NAANCTGGTG GNNCCGCNGN
            961 CNTCCNCTAN TNNTNNGNTA NAGTAAAAAN TANNNNNANA GCCNNNGNCC NNNNNNNNNN
          //
          Primer: IRES-F
          Region: IRES2,BSD,PRE
          Sequence file: RDB19558_B2C2c.seq check
          >D08711A4_B2C2_1_IRES-F_E12_15_ABI24.ab1
              1 NNNNNNNNNN NNNNNTTNNG TCGAGGTTAN NAAACGTCTA GGCCCCCCGA ACCNCGGGGA
             61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCATGGCC AAGCCTTTGT
            121 CTCAAGAAGA ATCCACCCTC ATTGAAAGAG CAACGGCTAC AATCAACAGC ATCCCCATCT
            181 CTGAAGACTA CAGCGTCGCC AGCGCAGCTC TCTCTAGCGA CGGCCGCATC TTCACTGGTG
            241 TCAATGTATA TCATTTTACT GGGGGACCTT GTGCAGAACT CGTGGTGCTG GGCACTGCTG
            301 CTGCTGCGGC AGCTGGCAAC CTGACTTGTA TCGTCGCGAT CGGAAATGAG AACAGGGGCA
            361 TCTTGAGCCC CTGCGGACGG TGCCGACAGG TGCTTCTCGA TCTGCATCCT GGGATCAAAG
            421 CCATAGTGAA GGACAGTGAT GGACAGCCGA CGGCAGTTGG GATTCGTGAA TTGCTGCCCT
            481 CTGGTTATGT GTGGGAGGGC TAAGTCGAGC CTCTAGAGTT AACATCGAGG GATCAAGCTT
            541 ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT
            601 GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT
            661 TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG
            721 GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC
            781 CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT NCGGGACTTT CGCTTTCCCC
            841 CTCCCTATTG NCACGGCGGA ACTCATCGCC GCCTGCCTTG NCCGCTGCTN GNNAGGGGCT
            901 CGNNTGNTGG GNNCTGANNN TTNCGNGGNG NTGNCGGGGA ANCTGANGNC CTTTCCATGG
            961 CTGNTCNNCN GNGNNNNCNN NNNNNNTCTG NNCGGGNNGT NNNNNTGNNN NGTCCCNTNN
          1021 NNNCNNNNNT NNNNCNNNNN NTNNNNNCNN NNNNNGNNGN NNNNNNNNN
          //
          Primer: HIV-B_R
          Region: HIV-1 psi,5'LTR,CMV pro.1,CMV en.1
          Sequence file: RDB19558_B2C2d.seq check
          >D08711A4_B2C2_1_HIV-B_R_C12_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNTTTTGGN GTACTCNCCA GTCGCCGCCC CTCGCCTCTT
             61 GCCGTGCGCG CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC
            121 GCTTTCAAGT CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC
            181 TGAGGGATCT CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC
            241 AAGGCAAGCT TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG
            301 CTCAGATCTG GTCTAACCAG AGAGACCCAG TACAGGCAAA ACGCGCTGCT TATATAGACC
            361 TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG CGGAGTTGTT ACGACATTTT
            421 GGAAAGTCCC GTTGATTTTG GTGCCAAAAC AAACTCCCAT TGACGTCAAT GGGGTGGAGA
            481 CTTGGAAATC CCCGTGAGTC AAACCGCTAT CCACGCCCAT TGATGTACTG CCAAAACCGC
            541 ATCACCATGG TAATAGCGAT GACTAATACG TAGATGTACT GCCAAGTAGG AAAGTCCCAT
            601 AAGGTCATGT ACTGGGCATA ATGCCAGGCG GGCCATTTAC CGTCATTGAC GTCAATAGGG
            661 GGCGTACTTG GCATATGATA CACTTGATGT ACTGCCAAGT GGGCAGTTTA CCGTAAATAC
            721 TCCACCCATT GACGTCAATG GAAAGTCCCT ATTGNCGTTA CTATGGGAAC ATACGTCATT
            781 ATTGACGTCN ATGGGCGGGG GTCGTTGGGC GGNCAGCCAG GCGGGCCATT TANCGTANGT
            841 TATGTAACGC GGAACTNCNT ATATGGGCTA TGAACTNATG ANCCCGTAAT TGANNACTAT
            901 TAATAACNAG TCNNTNANCN NTGTCNNCGC GNATNTCTGG NCNNTACNTC NNNAANNANC
          961 NNAAAANGNN TNNNCNNNAN NNNANNNNNC NNGNNNNGNC NGNNNNNNNN NN
          //
          Primer: bGH_rev3
          Region: bGH pA,3'LTR
          Sequence file: RDB19558_B2C2e.seq check
          >D08711A4_B2C2_1_bGH_rev3_G12_21_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGG NGGNNGGGNA NACNACAGAT GGCTGGCAAC TAGAAGGCAC
             61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA
            121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA
            181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT
            241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG
            301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT
            361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT
            421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT
            481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT
            541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC
            601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG
            661 GTACCTGANG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC
            721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCNTGTTTT TCTAGGTCTC
            781 GACGGTATCG ATGCGGGGAG GCGGNCCAAA GGGANNNCCG ANTCGTCTGA NGGCNNANNN
            841 NAANANGCGG AANANGNCNC ANANCCNGNA GCNNNCNCGG GNNNGAANGN CCGCTGNNNT
            901 GANGGNCNAN NGNNNGTANC ANANNNNNNN CCNCGCANAA NCCNNNNGGN ANCNCNNNNA
          961 NCANNNNTNN NANNNNNNNC NGCTTNCCCN NNNNNCCNNN NNNNNNN
          //
          Primer: RRE_F1
          Region: RRE,cPPT_CTS
          Sequence file: RDB19558_B2C2f.seq check
          >D08711A4_B2C2_1_RRE_F1_D12_12_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN ANATTTGCTG ANGGCTATTG AGGCGCAACA
             61 GCATCTGTTG CAACTCACAG TCTGGGGCAT CAAGCAGCTC CAGGCAAGAA TCCTGGCTGT
            121 GGAAAGATAC CTAAAGGATC AACAGCTCCT GGGGATTTGG GGTTGCTCTG GAAAACTCAT
            181 TTGCACCACT GCTGTGCCTT GGAATGCTAG TTGGAGTAAT AAATCTCTGG AACAGATTTG
            241 GAATCACACG ACCTGGATGG AGTGGGACAG AGAAATTAAC AATTACACAA GCTTAATACA
            301 CTCCTTAATT GAAGAATCGC AAAACCAGCA AGAAAAGAAT GAACAAGAAT TATTGGAATT
            361 AGATAAATGG GCAAGTTTGT GGAATTGGTT TAACATAACA AATTGGCTGT GGTATATAAA
            421 ATTATTCATA ATGATAGTAG GAGGCTTGGT AGGTTTAAGA ATAGTTTTTG CTGTACTTTC
            481 TATAGTGAAT AGAGTTAGGC AGGGATATTC ACCATTATCG TTTCAGACCC ACCTCCCAAC
            541 CCCGAGGGGA CCCGACAGGC CCGAAGGAAT AGAAGAAGAA GGTGGAGAGA GAGACAGAGA
            601 CAGATCCATT CGATTAGTGA ACGGATCTAC AAATGGCAGT ATTCATCCAC AATTTTAAAA
            661 GAAAAGGGGG GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG
            721 ACATACAAAC TAAAGAATTA CNAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT
            781 ACAGGGACAG CAGAAATTCA CTTTGAATTA ATTCGATTAA TAGTAATCAA TTACGGGGTC
            841 ATNNNTTCAT AGCCCATATA TGGAGTTCCG CGTTNCATAA CTTACNGNNA ATGGCCCGCC
            901 NNGCTGANCN NCCNACGACC CCNGCCCATN GANGNCAATN ATGACGTANG TNNCCNTAGT
            961 ANCGCCNANN NGGGNNTTTN CATTGACNNC NANGGNNGGN NTATTTNNNN NNAANNNGNC
          1021 NNNNNNNNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40 pro_ori,EM7 pro,Zeo
          Sequence file: RDB19558_B2C2g.seq check
          >D08711A4_B2C2_1_SV40pro_ori_F_A02_02_ABI24.ab1
              1 NNNNNNNNNN NGNNNNNGNC GCCTCTGCCT CTGANCTATT CCNGAAGTAG TGAGGAGGCT
             61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT
            121 CTGATCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA
            181 CAAGGTGAGG AACTAAACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG
            241 CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT
            301 GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA
            361 GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT
            421 GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT
            481 GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA
            541 CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC
            601 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT
            661 CCTCCAGCGC GGGGNTCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC
            721 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
            781 ACTGCATTCT AGTTGTGGNT TGNCCAAACT CNTCNATGNA TCTTATCATG TCTGTNTNNC
            841 GTCNANCTCT ANCTAGANCT NNNNNAATCA TGGNCATAGC TGNTTCCTGN GTGAAATTGN
            901 TATCCNCTCN CNNTTCCNCN CNNNATACNA NCNNNNNNCA TAAAGNGNAA AGNCNGGGGN
            961 NGNCNNNNGN NNGNNCNNNC TNNNNNNNNN TNNNNNNNNG CTNNNNNNCN GCNTNNNNNN
          1021 NNGNNANNNN NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Sasaki, M., SARS-CoV-2 variants with mutations at the S1/S2 cleavage site are generated in vitro during propagation in TMPRSS2-deficient cells. PLoS Pathog. 17 (1): e1009233 (2021). PMID 33476327.

          2022.08.18

          GNP_filter3_RDBDEP_html_220812.pl