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CSII-CMV-TMPRSS11E-IRES2-Bsd

Lentivirus expression vector of human TMPRSS11E, CMV promoter.

Catalog number RDB19556
Resource name CSII-CMV-TMPRSS11E-IRES2-Bsd
Clone info. Lentivirus expression vector of human transmembrane serine protease 11E (TMPRSS11E), CMV promoter.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. Expression was confirmed by the depositor with immunoblotting.
Vector backbone CSII-CMV-MCS-IRES2-Bsd (plasmid)
Size of vector backbone 9.5 kb
Selectable markers Ampicillin (E. coli), Bsd (mammalian cell)
Growth remarks Use NEBstable or equivalent to amplify this clone. 30 degC is fine. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name human TMPRSS11E cDNA
Depositor|Developer Sasaki, Michihito |
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External Database
human TMPRSS11E

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kishimoto, M. et al., Viruses, 13 (3): 384, 2021)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kishimoto, M. et al., Viruses, 13 (3): 384, 2021)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB19556 CSII-CMV-TMPRSS11E-IRES2-Bsd Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Original reference

          original Kishimoto, M., TMPRSS11D and TMPRSS13 Activate the SARS-CoV-2 Spike Protein. Viruses 13 (3): 384 (2021). PMID 33671076.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Kishimoto, M., TMPRSS11D and TMPRSS13 Activate the SARS-CoV-2 Spike Protein. Viruses 13 (3): 384 (2021). PMID 33671076.

          2022.08.18

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