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CSII-CMV-TMPRSS11D-IRES2-Bsd (#RDB19555)

Lentivirus expression vector of human TMPRSS11D, CMV promoter.

Clone info. Lentivirus expression vector of human transmembrane serine protease 11D (TMPRSS11D), CMV promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone CSII-CMV-MCS-IRES2-Bsd (RDB04385) (plasmid)
Size of vector backbone 9.5 kb
Selectable markers Ampicillin (E. coli), Bsd (lentivirus transfection). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth remarks Use NEBstable or equivalent to amplify this clone. 30 degC is fine. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name human TMPRSS11D cDNA
Depositor|Developer Sasaki, Michihito |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kishimoto, M. et al., Viruses, 13 (3): 384, 2021)
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kishimoto, M. et al., Viruses, 13 (3): 384, 2021)。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19555 CSII-CMV-TMPRSS11D-IRES2-Bsd Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The CSII-CMV-TMPRSS11D-IRES2-Bsd was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19555).

Reference section:

Kishimoto, M., Uemura. K., Sanaki, T., Sato, A., Hall, W.W., Kariwa, H., Orba, Y., Sawa, H., Sasaki, M., TMPRSS11D and TMPRSS13 Activate the SARS-CoV-2 Spike Protein. Viruses 13 (3): 384 (2021). PMID 33671076. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Kishimoto, M., TMPRSS11D and TMPRSS13 Activate the SARS-CoV-2 Spike Protein. Viruses 13 (3): 384 (2021). PMID 33671076. [link to RRC of NBRP]