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pEU-E01-NLRP3-FL-Bls (#RDB19445)

A wheat germ cell-free expression plasmid of the Biotin-tagged NLRP3 protein.

Clone info. A wheat germ cell-free expression plasmid of the Biotin-tagged Nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-3 (NLRP3) protein (biotin ligation site (Bls) amino acid sequence: GLNDIFEAQKIEWHE). In cell-free reconstituted NLRP3 innflammasome system, the purified product can be a donor of amplified luminescence proximity homogeneous assay (ALPHA) process in vitro. See the NLRP3-FL-Btn protein construct at Fig. 1 in Kaneko, N. et al. Sci. Rep. 10 (1): 13562, 2020.
Comment If you use a QIAGEN kit, it is recommended that you use the Plasmid Midi or Maxi Kit. The use of miniprep kits, including products from other companies, is not recommended. Expression was confirmed by the depositor with western blotting.
Vector backbone pEU-E01-GW-Bls (plasmid)
Size of vector backbone 5.0 kb
Selectable markers Ampicillin
Growth remarks Stbl2 is recommended E. coli for plamid amplication.
Gene/insert name human NLRP3 cDNA
Depositor|Developer Masumoto, Junya |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kaneko, N. et al., Sci. Rep. 10 (1): 13562, 2020)
Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kaneko, N. et al., Sci. Rep. 10 (1): 13562, 2020)。
備考
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19445 pEU-E01-NLRP3-FL-Bls Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pEU-E01-NLRP3-FL-Bls was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19445).

Reference section:

Kaneko, N., Kurata, M., Yamamoto, T., Shigemura, T., Agematsu, K., Yamazaki, T., Takeda, H., Sawasaki, T., Koga, T., Kawakami, A., Yachie, A., Migita, K., Yoshiura, K.I., Urano, T., Masumoto, J., KN3014, a piperidine-containing small compound, inhibits auto-secretion of IL-1β from PBMCs in a patient with Muckle-Wells syndrome. Sci. Rep. 10 (1): 13562 (2020). PMID 32782316. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Kaneko, N., KN3014, a piperidine-containing small compound, inhibits auto-secretion of IL-1β from PBMCs in a patient with Muckle-Wells syndrome. Sci. Rep. 10 (1): 13562 (2020). PMID 32782316. [link to RRC of NBRP]
reference Kaneko, N., Poly (I:C) and hyaluronic acid directly interact with NLRP3, resulting in the assembly of NLRP3 and ASC in a cell-free system. Eur. J. Inflamm. 15 (2): 85-97 (2017).