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pROSA26CAG-GFP-tdsRed

Flox expression vector of EGFP and tdsRed under the control of the CAG promoter used for knocking in mouse ROSA26 region.

Catalog number RDB19441
Resource name pROSA26CAG-GFP-tdsRed
Alternative name pROSA26CAG-GFP-tdDsRed
Clone info. Flox expression vector of EGFP and tdsRed (tandem version of dimeric DsRed) under the control of the CAG promoter used for knocking in ROSA26 region in mouse genome. See Fig.1 of Hasegawa, Y.et al. Exp. Anim. 62 (4): 295-304, 2013.
Comment Expression was confirmed by the deposiotor with microscopy.
Vector backbone pBluescript (plasmid)
Selectable markers Ampicillin
Gene/insert name mouse Rosa26 genomic DNA; CAG promoter; Aequorea victoria GFP cDNA (EGFP); Discosoma sp. RFP cDNA (DsRed1); S. cerevisiae FRT recombination sequences; E. coli neomycin resistance gene; Corynebacterium diphtheriae Diphtheria toxin flagment A gene (DTA)
Depositor|Developer Miwa, Yoshihiro |
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External Database
mouse ROSA26 Corynebacterium diphtheriae Dipbachtheria toxin A

        Reference sequence
          
         

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Hasegawa, Y. et. al., Exp.Anim. 62(4), 295-304, 2013).
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hasegawa, Y. et. al., Exp.Anim. 62(4), 295-304, 2013)。
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        備考 タンパク質毒素遺伝子です (diphtheria toxin A subunit)。EK1 (組換え大腸菌) 以外の使用は、「二種省令別表第一第一号ト」とポジションペーパーを参照してください。

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        RDB19441 pROSA26CAG-GFP-tdsRed Under QC test. Please contact us. DNA solution

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        How to cite this biological resource

        Materials & Methods section:

        The pROSA26CAG-GFP-tdsRed was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19441).

        Reference section:

        Hasegawa, Y., Daitoku, Y., Sekiguchi, K., Tanimoto, Y., Mizuno-Iijima, S., Mizuno, S., Kajiwara, N., Ema, M., Miwa, Y., Mekada, K., Yoshiki, A., Takahashi, S., Sugiyama, F., Yagami, K., Novel ROSA26 Cre-reporter knock-in C57BL/6N mice exhibiting green emission before and red emission after Cre-mediated recombination. Exp. Anim. 62 (4): 295-304 (2013). PMID 24172193. [link to RRC of NBRP]

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        QC test results

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        References

        Original, user report and related articles

        original Hasegawa, Y., Novel ROSA26 Cre-reporter knock-in C57BL/6N mice exhibiting green emission before and red emission after Cre-mediated recombination. Exp. Anim. 62 (4): 295-304 (2013). PMID 24172193. [link to RRC of NBRP]

        2023.12.12

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