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pROSA26CAG-GFP-tdsRed

Flox expression vector of EGFP and tdsRed under the control of the CAG promoter used for knocking in mouse ROSA26 region.

Catalog number RDB19441
Resource name pROSA26CAG-GFP-tdsRed
Alternative name pROSA26CAG-GFP-tdDsRed
Clone info. Flox expression vector of EGFP and tdsRed (tandem version of dimeric DsRed) under the control of the CAG promoter used for knocking in ROSA26 region in mouse genome. See Fig.1 of Hasegawa, Y.et al. Exp. Anim. 62 (4): 295-304, 2013.
Comment Expression was confirmed by the deposiotor with microscopy.
Vector backbone pBluescript (plasmid)
Selectable markers Ampicillin
Gene/insert name mouse Rosa26 genomic DNA; CAG promoter; Aequorea victoria GFP cDNA (EGFP); Discosoma sp. RFP cDNA (DsRed1); S. cerevisiae FRT recombination sequences; E. coli neomycin resistance gene; Corynebacterium diphtheriae Diphtheria toxin flagment A gene (DTA)
Depositor|Developer Miwa, Yoshihiro |
Other clones in our bank
External Database
mouse ROSA26 Corynebacterium diphtheriae Dipbachtheria toxin A
        Reference sequence
          
         

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Hasegawa, Y. et. al., Exp.Anim. 62(4), 295-304, 2013).
        Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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        This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        提供案内 (日本国内) [open/close]

        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hasegawa, Y. et. al., Exp.Anim. 62(4), 295-304, 2013)。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
        提供依頼書 [Word]
        Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。
        備考 タンパク質毒素遺伝子です (diphtheria toxin A subunit)。EK1 (組換え大腸菌) 以外の使用は、「二種省令別表第一第一号ト」とポジションペーパーを参照してください。

        Catalog # Resource name Availability Shipping form Fee (non-profit org.)
        RDB19441 pROSA26CAG-GFP-tdsRed Under QC test. Please contact us. DNA solution

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        References and tips

        Original reference

        original Hasegawa, Y., Novel ROSA26 Cre-reporter knock-in C57BL/6N mice exhibiting green emission before and red emission after Cre-mediated recombination. Exp. Anim. 62 (4): 295-304 (2013). PMID 24172193.

        Further references such as user reports and related articles (go to bottom)

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        QC test results

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        References

        Original, user report and related articles

        original Hasegawa, Y., Novel ROSA26 Cre-reporter knock-in C57BL/6N mice exhibiting green emission before and red emission after Cre-mediated recombination. Exp. Anim. 62 (4): 295-304 (2013). PMID 24172193.

        2023.01.23

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