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pROSA26CAG-GFP-tdsRed (#RDB19441)

Flox expression vector of EGFP and tdsRed under the control of the CAG promoter used for knocking in mouse ROSA26 region.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Hasegawa, Y., Exp. Anim. 62 (4): 295-304 (2013). PMID 24172193. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name pROSA26CAG-GFP-tdDsRed
Clone info. Flox expression vector of EGFP and tdsRed (tandem version of dimeric DsRed) under the control of the CAG promoter used for knocking in ROSA26 region in mouse genome. See Fig.1 of Hasegawa, Y.et al. Exp. Anim. 62 (4): 295-304, 2013.
Comment Expression was confirmed by the deposiotor with microscopy.
Vector backbone pBluescript (plasmid)
Selectable markers Ampicillin
Growth conditions LB+Amp, 37oC
Gene/insert name mouse Rosa26 genomic DNA; CAG promoter; Aequorea victoria GFP cDNA (EGFP); Discosoma sp. RFP cDNA (DsRed1); S. cerevisiae FRT recombination sequences; E. coli neomycin resistance gene; Corynebacterium diphtheriae Diphtheria toxin flagment A gene (DTA)
Depositor|Developer Miwa, Yoshihiro |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Hasegawa, Y. et. al., Exp.Anim. 62(4), 295-304, 2013).
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hasegawa, Y. et. al., Exp.Anim. 62(4), 295-304, 2013)。
備考 タンパク質毒素遺伝子です (diphtheria toxin A subunit)。EK1 (組換え大腸菌) 以外の使用は、「二種省令別表第一第一号ト」とポジションペーパーを参照してください。

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RDB19441 pROSA26CAG-GFP-tdsRed DNA solution

How to cite this biological resource

Materials & Methods section:

The pROSA26CAG-GFP-tdsRed was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19441).

Reference section:

Hasegawa, Y., Daitoku, Y., Sekiguchi, K., Tanimoto, Y., Mizuno-Iijima, S., Mizuno, S., Kajiwara, N., Ema, M., Miwa, Y., Mekada, K., Yoshiki, A., Takahashi, S., Sugiyama, F., Yagami, K., Novel ROSA26 Cre-reporter knock-in C57BL/6N mice exhibiting green emission before and red emission after Cre-mediated recombination. Exp. Anim. 62 (4): 295-304 (2013). PMID 24172193. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hasegawa, Y., Novel ROSA26 Cre-reporter knock-in C57BL/6N mice exhibiting green emission before and red emission after Cre-mediated recombination. Exp. Anim. 62 (4): 295-304 (2013). PMID 24172193. [PubMed] [Article] [RRC of NBRP]