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pET-scFv-GFPA36

Bacterial expression vector of mouse scFv-A36 that binds to C2A domain of SytI/II with T7-, EGFP- and 6xHis-tags.

Catalog number RDB19422
Resource name pET-scFv-GFPA36
Clone info. Bacterial expression vector of mouse scFv-A36 that binds to C2A domain of synaptotagmin I and II (Syt I/II-C2A). The T7-tag and EGFP-tag at N-terminus and 6xHis-tag at C-terminus. This plasmid is for an ultra-stable cytoplasmic antibody (STAND) study. See the scFv protein construct at Fig. 1 in Kabayama N. et al. Nat. Commun. 11 (1): 336, 2020.
Comment Protein can be induced with 0.1 mM IPTG for 4 hr at 30°C in E. coli (BL21 [DE3]) expressing thioredoxin (Trx) from pT-Trx. Expression was confirmed by the depositor with Western blotting.
Vector backbone pET3a (plasmid)
Size of vector backbone 4.6 kb
Selectable markers Ampicillin
Gene/insert name mouse scFv cDNA
Reference of insert sequence AB472376 (DDBJ accession number)
Depositor|Developer Mikoshiba, Katsuhiko | Kabayama, Hiroyuki |
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External Database
mouse scFv

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR RIKEN BRC will provide the resources with the consent of the inventors (Katsuhiko Mikoshiba and Hiroyuki Kabayama). The publication, conditions of use, and attribution of research results using the resources, including research papers, patent applications, and business use, shall be separately stipulated in a contract, memorandum, or agreement after consultation with the inventors. Provision to third parties is prohibited.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            Approval form by depositor [Word]
            Remarks
            Please obtain written approval of the depositor.
            提供案内 (日本国内) [open/close]

            提供条件 理研BRCは、発明者(御子柴克彦、樺山博之)の了解を得た上でリソースを提供する。論文発表、特許出願、事業利用を含め、それを用いた研究成果の公表、利用条件及び帰属は、別途、発明者との協議の上、契約書、覚書または同意書に定める。第三者への提供を禁ずる。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
            寄託者による提供承諾書 [Word]
            備考
            寄託者の書面による提供承諾が必要です。

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB19422 pET-scFv-GFPA36 Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Electronic file

            Original reference

            original Kabayama, H., An ultra-stable cytoplasmic antibody engineered for in vivo applications. Nat. Commun. 11 (1): 336 (2020). PMID 31953402.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            References

            Original, user report and related articles

            original Kabayama, H., An ultra-stable cytoplasmic antibody engineered for in vivo applications. Nat. Commun. 11 (1): 336 (2020). PMID 31953402.

            2022.07.13

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