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pET-scFv-GFPA36

Bacterial expression vector of mouse scFv-A36 that binds to C2A domain of SytI/II with T7-, EGFP- and 6xHis-tags.

Catalog number RDB19422
Resource name pET-scFv-GFPA36
Clone info. Bacterial expression vector of mouse scFv-A36 that binds to C2A domain of synaptotagmin I and II (Syt I/II-C2A). The T7-tag and EGFP-tag at N-terminus and 6xHis-tag at C-terminus. This plasmid is for an ultra-stable cytoplasmic antibody (STAND) study. See the scFv protein construct at Fig. 1 in Kabayama N. et al. Nat. Commun. 11 (1): 336, 2020.
Comment Protein can be induced with 0.1 mM IPTG for 4 hr at 30°C in E. coli (BL21 [DE3]) expressing thioredoxin (Trx) from pT-Trx. Expression was confirmed by the depositor with Western blotting.
Vector backbone pET3a (plasmid)
Size of vector backbone 4.6 kb
Selectable markers Ampicillin
Gene/insert name mouse scFv cDNA
Reference of insert sequence AB472376 (DDBJ accession number)
Depositor|Developer Mikoshiba, Katsuhiko | Kabayama, Hiroyuki |
Other clones in our bank
External Database
mouse scFv
            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution RIKEN BRC will provide the resources with the consent of the inventors (Katsuhiko Mikoshiba and Hiroyuki Kabayama). The publication, conditions of use, and attribution of research results using the resources, including research papers, patent applications, and business use, shall be separately stipulated in a contract, memorandum, or agreement after consultation with the inventors. Provision to third parties is prohibited.
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            RDB19422 pET-scFv-GFPA36 Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Original reference

            original Kabayama, H., An ultra-stable cytoplasmic antibody engineered for in vivo applications. Nat. Commun. 11 (1): 336 (2020). PMID 31953402.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Kabayama, H., An ultra-stable cytoplasmic antibody engineered for in vivo applications. Nat. Commun. 11 (1): 336 (2020). PMID 31953402.

            2023.04.06

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