Resource data sheet
DNA Bank Top

pFX-H2B-SECFP (#RDB19414)

Expression vector of SECFP with nucleus-targeting sequence (human H2B) in mammalian cells.


Drawn by SnapGene® software
Sequence (full) RDB19414hts01.seq
Publication Kashiwagi, S., Cell Struct. Funct. 44 (2): 183-194 (2019). [link to RRC of NBRP]
Test sheet RDB19414_B1Kfp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Expression vector of SECFP (super-enhanced cyan-emitting fluorescent protein) with nucleus-targeting sequence (human histone 2B; H2B) in mammalian cells.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pFX (plasmid)
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Growth conditions LB+Kanamycin, 37oC
Gene/insert name human H2BC11 cDNA
Depositor|Developer Ohba, Yusuke |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kashiwagi, S. et al., Cell Struct. Funct. 44 (2): 183-194, 2019)
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, an acknowledgement to Dr. Miyawaki is requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kashiwagi, S. et al., Cell Struct. Funct. 44 (2): 183-194, 2019)。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, an acknowledgement to Dr. Miyawaki is requested.

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19414 pFX-H2B-SECFP DNA solution

How to cite this biological resource

Materials & Methods section:

The pFX-H2B-SECFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19414).

Reference section:

Kashiwagi, S., Fujioka, Y., Satoh, A.O., Yoshida, A., Fujioka, M., Nepal, P., Tsuzuki, A., Aoki, O., Paudel, S., Sasajima, H., Ohba, Y., Folding Latency of Fluorescent Proteins Affects the Mitochondrial Localization of Fusion Proteins. Cell Struct. Funct. 44 (2): 183-194 (2019). PMID 31735740. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kashiwagi, S., Folding Latency of Fluorescent Proteins Affects the Mitochondrial Localization of Fusion Proteins. Cell Struct. Funct. 44 (2): 183-194 (2019). PMID 31735740. [link to RRC of NBRP]