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pYU99 pRS316-ADH1p-mCherry-MCS-CYC1ter (#RDB19395)

Yeast expression vector with the ADH1 promoter and CYC1 terminator. URA3 marker. mCherry-tag at N-terminus.

Alternative name pYU99
Clone info. Yeast expression vector with the ADH1 promoter and CYC1 terminator. URA3 marker. mCherry-tag at N-terminus.
Vector backbone pRS316 (plasmid)
Size of vector backbone 4.9 kb
Selectable markers Ampicillin (E. coli), URA3 (S. cerevisiae)
Depositor|Developer Tamura, Yasushi |
 
Sequence (full) RDB19395zzt01.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. (Tashiro, S. et al., Front. Cell. Dev. Biol. 8: 571388, 2020)
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19395 pYU99 pRS316-ADH1p-mCherry-MCS-CYC1ter DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pYU99 pRS316-ADH1p-mCherry-MCS-CYC1ter was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19395).

Reference section:

Tashiro, S., Kakimoto, Y., Shinmyo, M., Fujimoto, S., Tamura, Y., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19395_B1K1p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R (Pr0800)
Region: T7 pro, insert 5' (ADH1 pro)
Sequence file: RDB19395_B1K1a.seq check
>D08418D5_B1K1_1_HB-NOS_R_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGCN NGGGTTTTCC CAGTCNCGAC
   61 GTTGTAAAAC GACGGCCAGT GNATTGTAAT ACGACTCACT ATAGGGCGAA TTGGAGCTCC
  121 GATTTTTTTC TAAACCGTGG AATATTTCGG ATATCCTTTT GTTGTTTCCG GGTGTACAAT
  181 ATGGACTTCC TCTTTTCTGG CAACCAAACC CATACATCGG GATTCCTATA ATACCTTCGT
  241 TGGTCTCCCT AACATGTAGG TGGCGGAGGG GAGATATACA ATAGAACAGA TACCAGACAA
  301 GACATAATGG GCTAAACAAG ACTACACCAA TTACACTGCC TCATTGATGG TGGTACATAA
  361 CGAACTAATA CTGTAGCCCT AGACTTGATA GCCATCATCA TATCGAAGTT TCACTACCCT
  421 TTTTCCATTT GCCATCTATT GAAGTAATAA TAGGCGCATG CAACTTCTTT TCTTTTTTTT
  481 TCTTTTCTCT CTCCCCCGTT GTTGTCTCAC CATATCCGCA ATGACAAAAA AATGATGGAA
  541 GACACTAAAG GAAAAAATTA ACGACAAAGA CAGCACCAAC AGATGTCGTT GTTCCAGAGC
  601 TGATGAGGGG TATCTCGAAG CACACGAAAC TTTTTCCTTC CTTCATTCAC GCACACTACT
  661 CTCTAATGAG CAACGGTATA CGGCCTTCCT TCCAGTTACT TGAATTTGAA ATAAAAAAAA
  721 GTTTGCTGTC TTGCTATCAA GTATAAATAG ACCTGCAATT ATTAATCTTT TGTTTCCTCG
  781 TCATTGTTCT CGTTCCCTTT CTTCCTTGTT TCTTTTTCTG CACAATATTT CNNGCTATAC
  841 CAAGCNTACA ATCNNCTATC TCATATACNG CGGNCGCATG GTGANCNANG GNNNANGANG
  901 ATAACATGGN CNTCNTCAAN GANTTCATGC NCNTNNANNN GCANNTGGNN GGGCTNCNNG
961 AACNGNNCNN NNNTNCNANN NCGANGGCNN NNGNNNNNNN NNN
//
Primer: pUC-CAPbs_up_F2 (Pr0739)
Region: T3 pro, insert 3' (CYC1 ter, MCS, mCherry 3')
Sequence file: RDB19395_B1K1b.seq check
>D08418D5_B1K1_1_pUC-CAPbs_up_F2_A02_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NCTTCCGGCT CNNATGTTGT
   61 GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA
  121 AGCTCGAAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGT ACCGGCCGCA AATTAAAGCC
  181 TTCGAGCGTC CCAAAACCTT CTCAAGCAAG GTTTTCAGTA TAATGTTACA TGCGTACACG
  241 CGTCTGTACA GAAAAAAAAG AAAAATTTGA AATATAAATA ACGTTCTTAA TACTAACATA
  301 ACTATAAAAA AATAAATAGG GACCTAGACT TCAGGTTGTC TAACTCCTTC CTTTTCGGTT
  361 AGAGCGGATG TGGGGGGAGG GCGTGAATGT AAGCGTGACA TAACTAATTA CATGACTCGA
  421 GGTCGACGGT ATCGATAAGC TTGATATCGA ATTCCTGCAG CCCGGGGGAT CCACTAGTCT
  481 TGTACAGCTC GTCCATGCCG CCGGTGGAGT GGCGGCCCTC GGCGCGTTCG TACTGTTCCA
  541 CGATGGTGTA GTCCTCGTTG TGGGAGGTGA TGTCCAACTT GATGTTGACG TTGTAGGCGC
  601 CGGGCAGCTG CACGGGCTTC TTGGCCTTGT AGGTGGTCTT GACCTCAGCG TCGTAGTGGC
  661 CGCCGTCCTT CAGCTTCAGC CTCTGCTTGA TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT
  721 ACATCCGCTC GGAGGAGGCC TCCCAGCCCA TGGTCTTCTT CTGCATNACG GGGCCGTCGG
  781 AGGGGAAGTT GGTGCCGCGC ANCTTCACCT TGTAGANGAN CTCGCCNTCC TGCANGGNNG
  841 AGTCCTGGGT CACGGTCACC ACNCCNCCNT CCTCNNANNN TCATCACGCG CTCCNNCTTG
  901 AAGCCNTCNG GGAANGNCAG CTTCAAGNAN TCNGGGNNGT NNNNNGGGTG CTTCACGNAN
  961 GCCTTGNANC CNTNNNTGAA CTGAGGGNNA GGNNGTCCCN NNNNNNNGGC AGGGGGNCNN
 1021 CNNNGGNNNN NNCANNNNNN NNNCNGNNNN CCNCNNNNGG NNGNNNNNGC CNNNNNNNNN
1081 N
//
Primer: URA3pro_F (Pr0170)
Region: URA3 pro, URA3
Sequence file: RDB19395_B1K1c.seq check
>D08418D5_B1K1_1_URA3pro_F_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNATTCTTAA CCCANNCTGC
   61 ACAGNAACAA AAACCTGCAG GAAACGAAGA TAAATCATGT CGAAAGCTAC ATATAAGGAA
  121 CGTGCTGCTA CTCATCCTAG TCCTGTTGCT GCCAAGCTAT TTAATATCAT GCACGAAAAG
  181 CAAACAAACT TGTGTGCTTC ATTGGATGTT CGTACCACCA AGGAATTACT GGAGTTAGTT
  241 GAAGCATTAG GTCCCAAAAT TTGTTTACTA AAAACACATG TGGATATCTT GACTGATTTT
  301 TCCATGGAGG GCACAGTTAA GCCGCTAAAG GCATTATCCG CCAAGTACAA TTTTTTACTC
  361 TTCGAAGACA GAAAATTTGC TGACATTGGT AATACAGTCA AATTGCAGTA CTCTGCGGGT
  421 GTATACAGAA TAGCAGAATG GGCAGACATT ACGAATGCAC ACGGTGTGGT GGGCCCAGGT
  481 ATTGTTAGCG GTTTGAAGCA GGCGGCAGAA GAAGTAACAA AGGAACCTAG AGGCCTTTTG
  541 ATGNNNNNAG AATTGTCATG CAAGGGCTCC CTATCTACTG GAGANNNNNN TAAGGGTACT
  601 GTTGACATTG CGAAGAGCGA CAAAGATTTT GTTATCGGCT TTATTGCTCA AAGAGACATG
  661 GGTGGAAGAG ATGAAGGTTA CGATTGGTTG ANTATGACAC CCGGTGTGGG TTTAGATGAC
  721 AAGGGAGACG CNTTGGGTCA ACAGTATAGA ACCGTGGATG ATGTGGTCTC TACAGGATCT
  781 GACNTTATTA TTGTTGNAAG ANGACTATTT GCNAAGGGAN NGGATGCTAN NGTANNNGGT
  841 GAACGTNACN GAAAAAGCNN GCTGGGAAGC ATATTTGANA NNNNGCNNNC CAGCAAAACT
  901 AAAAAAANTG TATTNNTNAG NNAANNGCAT GNTATNCTAA ACNNNACAAA NNTANNNNNT
  961 NCNNTTTNNA NNTNATANNC NNNTNNTNNN NNNTNNGCNG NNNNNNAANN NNNCNNNNNN
1021 NNNN
//
Primer: ADH1pro_F (Pr0450)
Region: insert mid (ADH1 pro, mCherry 5')
Sequence file: RDB19395_B1K1d.seq check
>D08418D5_B1K1_1_ADH1pro_F_E03_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN TTTGNNNNNN GTNNTTGTTC TCGTTCCCTT TCTTCCTTGT
   61 TTCTTTTTCT GCACAATATT TCAAGCTATA CCAAGCATAC AATCAACTAT CTCATATACA
  121 GCGGCCGCAT GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC
  181 GCTTCAAGGT GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG
  241 GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC
  301 CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG
  361 TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT
  421 GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC
  481 TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG
  541 GCCCCGTAAT GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG
  601 AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT
  661 ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT
  721 ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCNNGGAAC
  781 AGTACGAACG CGCCGAGGGC CGCCACTCCA NCNGCGGCAT GGACGANCTG TNCAAGACTA
  841 GTGGATCCCC CGGGCTGCAN GAANTCGATA TCAAGCTTAT CGATACCGNC NANCTCNAGT
  901 CATGTAATTA GTTATGTCAN NCTNACNTNC NCGCCCNNCC CCCACATNCG CTCTNACCGA
  961 AANNGAAGGA GTTAGANNAN NTGAAGNCTA NGNNCCNNTT ATTTTTTTAN ANNNNNGNTN
1021 GNNNNNNNAC GNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450. [link to RRC of NBRP]