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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pYU99 pRS316-ADH1p-mCherry-MCS-CYC1ter

Yeast expression vector with the ADH1 promoter and CYC1 terminator. URA3 marker. mCherry-tag at N-terminus.

Catalog number RDB19395
Resource name pYU99 pRS316-ADH1p-mCherry-MCS-CYC1ter
Alternative name pYU99
Clone info. Yeast expression vector with the ADH1 promoter and CYC1 terminator. URA3 marker. mCherry-tag at N-terminus.
Vector backbone pRS316 (plasmid)
Size of vector backbone 4.9 kb
Selectable markers Ampicillin (E. coli), URA3 (S. cerevisiae)
Depositor|Developer Tamura, Yasushi |
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External Database

            Reference sequence
              
             
            Sequence (full) RDB19395zzt01.seq compiled by us

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. (Tashiro, S. et al., Front. Cell. Dev. Biol. 8: 571388, 2020)
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            This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
            Remarks
            This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. A part of license fees is required. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
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            提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Tashiro, S. et al., Front. Cell. Dev. Biol. 8: 571388, 2020)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
            備考
            Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。ライセンス料相当額を負担して頂きます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

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            RDB19395 pYU99 pRS316-ADH1p-mCherry-MCS-CYC1ter DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB19395_B1K1p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: HB-NOS_R
            Region: T7 pro, insert 5' (ADH1 pro)
            Sequence file: RDB19395_B1K1a.seq check
            >D08418D5_B1K1_1_HB-NOS_R_A01_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGCN NGGGTTTTCC CAGTCNCGAC
               61 GTTGTAAAAC GACGGCCAGT GNATTGTAAT ACGACTCACT ATAGGGCGAA TTGGAGCTCC
              121 GATTTTTTTC TAAACCGTGG AATATTTCGG ATATCCTTTT GTTGTTTCCG GGTGTACAAT
              181 ATGGACTTCC TCTTTTCTGG CAACCAAACC CATACATCGG GATTCCTATA ATACCTTCGT
              241 TGGTCTCCCT AACATGTAGG TGGCGGAGGG GAGATATACA ATAGAACAGA TACCAGACAA
              301 GACATAATGG GCTAAACAAG ACTACACCAA TTACACTGCC TCATTGATGG TGGTACATAA
              361 CGAACTAATA CTGTAGCCCT AGACTTGATA GCCATCATCA TATCGAAGTT TCACTACCCT
              421 TTTTCCATTT GCCATCTATT GAAGTAATAA TAGGCGCATG CAACTTCTTT TCTTTTTTTT
              481 TCTTTTCTCT CTCCCCCGTT GTTGTCTCAC CATATCCGCA ATGACAAAAA AATGATGGAA
              541 GACACTAAAG GAAAAAATTA ACGACAAAGA CAGCACCAAC AGATGTCGTT GTTCCAGAGC
              601 TGATGAGGGG TATCTCGAAG CACACGAAAC TTTTTCCTTC CTTCATTCAC GCACACTACT
              661 CTCTAATGAG CAACGGTATA CGGCCTTCCT TCCAGTTACT TGAATTTGAA ATAAAAAAAA
              721 GTTTGCTGTC TTGCTATCAA GTATAAATAG ACCTGCAATT ATTAATCTTT TGTTTCCTCG
              781 TCATTGTTCT CGTTCCCTTT CTTCCTTGTT TCTTTTTCTG CACAATATTT CNNGCTATAC
              841 CAAGCNTACA ATCNNCTATC TCATATACNG CGGNCGCATG GTGANCNANG GNNNANGANG
              901 ATAACATGGN CNTCNTCAAN GANTTCATGC NCNTNNANNN GCANNTGGNN GGGCTNCNNG
            961 AACNGNNCNN NNNTNCNANN NCGANGGCNN NNGNNNNNNN NNN
            //
            Primer: pUC-CAPbs_up_F2
            Region: T3 pro, insert 3' (CYC1 ter, MCS, mCherry 3')
            Sequence file: RDB19395_B1K1b.seq check
            >D08418D5_B1K1_1_pUC-CAPbs_up_F2_A02_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NCTTCCGGCT CNNATGTTGT
               61 GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA
              121 AGCTCGAAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGT ACCGGCCGCA AATTAAAGCC
              181 TTCGAGCGTC CCAAAACCTT CTCAAGCAAG GTTTTCAGTA TAATGTTACA TGCGTACACG
              241 CGTCTGTACA GAAAAAAAAG AAAAATTTGA AATATAAATA ACGTTCTTAA TACTAACATA
              301 ACTATAAAAA AATAAATAGG GACCTAGACT TCAGGTTGTC TAACTCCTTC CTTTTCGGTT
              361 AGAGCGGATG TGGGGGGAGG GCGTGAATGT AAGCGTGACA TAACTAATTA CATGACTCGA
              421 GGTCGACGGT ATCGATAAGC TTGATATCGA ATTCCTGCAG CCCGGGGGAT CCACTAGTCT
              481 TGTACAGCTC GTCCATGCCG CCGGTGGAGT GGCGGCCCTC GGCGCGTTCG TACTGTTCCA
              541 CGATGGTGTA GTCCTCGTTG TGGGAGGTGA TGTCCAACTT GATGTTGACG TTGTAGGCGC
              601 CGGGCAGCTG CACGGGCTTC TTGGCCTTGT AGGTGGTCTT GACCTCAGCG TCGTAGTGGC
              661 CGCCGTCCTT CAGCTTCAGC CTCTGCTTGA TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT
              721 ACATCCGCTC GGAGGAGGCC TCCCAGCCCA TGGTCTTCTT CTGCATNACG GGGCCGTCGG
              781 AGGGGAAGTT GGTGCCGCGC ANCTTCACCT TGTAGANGAN CTCGCCNTCC TGCANGGNNG
              841 AGTCCTGGGT CACGGTCACC ACNCCNCCNT CCTCNNANNN TCATCACGCG CTCCNNCTTG
              901 AAGCCNTCNG GGAANGNCAG CTTCAAGNAN TCNGGGNNGT NNNNNGGGTG CTTCACGNAN
              961 GCCTTGNANC CNTNNNTGAA CTGAGGGNNA GGNNGTCCCN NNNNNNNGGC AGGGGGNCNN
             1021 CNNNGGNNNN NNCANNNNNN NNNCNGNNNN CCNCNNNNGG NNGNNNNNGC CNNNNNNNNN
            1081 N
            //
            Primer: URA3pro_F
            Region: URA3 pro, URA3
            Sequence file: RDB19395_B1K1c.seq check
            >D08418D5_B1K1_1_URA3pro_F_C01_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNATTCTTAA CCCANNCTGC
               61 ACAGNAACAA AAACCTGCAG GAAACGAAGA TAAATCATGT CGAAAGCTAC ATATAAGGAA
              121 CGTGCTGCTA CTCATCCTAG TCCTGTTGCT GCCAAGCTAT TTAATATCAT GCACGAAAAG
              181 CAAACAAACT TGTGTGCTTC ATTGGATGTT CGTACCACCA AGGAATTACT GGAGTTAGTT
              241 GAAGCATTAG GTCCCAAAAT TTGTTTACTA AAAACACATG TGGATATCTT GACTGATTTT
              301 TCCATGGAGG GCACAGTTAA GCCGCTAAAG GCATTATCCG CCAAGTACAA TTTTTTACTC
              361 TTCGAAGACA GAAAATTTGC TGACATTGGT AATACAGTCA AATTGCAGTA CTCTGCGGGT
              421 GTATACAGAA TAGCAGAATG GGCAGACATT ACGAATGCAC ACGGTGTGGT GGGCCCAGGT
              481 ATTGTTAGCG GTTTGAAGCA GGCGGCAGAA GAAGTAACAA AGGAACCTAG AGGCCTTTTG
              541 ATGNNNNNAG AATTGTCATG CAAGGGCTCC CTATCTACTG GAGANNNNNN TAAGGGTACT
              601 GTTGACATTG CGAAGAGCGA CAAAGATTTT GTTATCGGCT TTATTGCTCA AAGAGACATG
              661 GGTGGAAGAG ATGAAGGTTA CGATTGGTTG ANTATGACAC CCGGTGTGGG TTTAGATGAC
              721 AAGGGAGACG CNTTGGGTCA ACAGTATAGA ACCGTGGATG ATGTGGTCTC TACAGGATCT
              781 GACNTTATTA TTGTTGNAAG ANGACTATTT GCNAAGGGAN NGGATGCTAN NGTANNNGGT
              841 GAACGTNACN GAAAAAGCNN GCTGGGAAGC ATATTTGANA NNNNGCNNNC CAGCAAAACT
              901 AAAAAAANTG TATTNNTNAG NNAANNGCAT GNTATNCTAA ACNNNACAAA NNTANNNNNT
              961 NCNNTTTNNA NNTNATANNC NNNTNNTNNN NNNTNNGCNG NNNNNNAANN NNNCNNNNNN
            1021 NNNN
            //
            Primer: ADH1pro_F
            Region: insert mid (ADH1 pro, mCherry 5')
            Sequence file: RDB19395_B1K1d.seq check
            >D08418D5_B1K1_1_ADH1pro_F_E03_05_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN TTTGNNNNNN GTNNTTGTTC TCGTTCCCTT TCTTCCTTGT
               61 TTCTTTTTCT GCACAATATT TCAAGCTATA CCAAGCATAC AATCAACTAT CTCATATACA
              121 GCGGCCGCAT GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC
              181 GCTTCAAGGT GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG
              241 GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC
              301 CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG
              361 TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT
              421 GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC
              481 TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG
              541 GCCCCGTAAT GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG
              601 AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT
              661 ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT
              721 ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCNNGGAAC
              781 AGTACGAACG CGCCGAGGGC CGCCACTCCA NCNGCGGCAT GGACGANCTG TNCAAGACTA
              841 GTGGATCCCC CGGGCTGCAN GAANTCGATA TCAAGCTTAT CGATACCGNC NANCTCNAGT
              901 CATGTAATTA GTTATGTCAN NCTNACNTNC NCGCCCNNCC CCCACATNCG CTCTNACCGA
              961 AANNGAAGGA GTTAGANNAN NTGAAGNCTA NGNNCCNNTT ATTTTTTTAN ANNNNNGNTN
            1021 GNNNNNNNAC GNNNNNNNNN N
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450.

            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl