Please review the QC test results indicated by icon below as well as clone information before placing your order.

pYU99 pRS316-ADH1p-mCherry-MCS-CYC1ter

Yeast expression vector with the ADH1 promoter and CYC1 terminator. URA3 marker. mCherry-tag at N-terminus.

Sequence (full)	RDB19395zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. (Tashiro, S. et al., Front. Cell. Dev. Biol. 8: 571388, 2020)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
Remarks	This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. A part of license fees is required. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Tashiro, S. et al., Front. Cell. Dev. Biol. 8: 571388, 2020)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
備考	Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。ライセンス料相当額を負担して頂きます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19395_B1K1p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R Region: T7 pro, insert 5' (ADH1 pro) Sequence file: RDB19395_B1K1a.seq
>D08418D5_B1K1_1_HB-NOS_R_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGCN NGGGTTTTCC CAGTCNCGAC    61 GTTGTAAAAC GACGGCCAGT GNATTGTAAT ACGACTCACT ATAGGGCGAA TTGGAGCTCC   121 GATTTTTTTC TAAACCGTGG AATATTTCGG ATATCCTTTT GTTGTTTCCG GGTGTACAAT   181 ATGGACTTCC TCTTTTCTGG CAACCAAACC CATACATCGG GATTCCTATA ATACCTTCGT   241 TGGTCTCCCT AACATGTAGG TGGCGGAGGG GAGATATACA ATAGAACAGA TACCAGACAA   301 GACATAATGG GCTAAACAAG ACTACACCAA TTACACTGCC TCATTGATGG TGGTACATAA   361 CGAACTAATA CTGTAGCCCT AGACTTGATA GCCATCATCA TATCGAAGTT TCACTACCCT   421 TTTTCCATTT GCCATCTATT GAAGTAATAA TAGGCGCATG CAACTTCTTT TCTTTTTTTT   481 TCTTTTCTCT CTCCCCCGTT GTTGTCTCAC CATATCCGCA ATGACAAAAA AATGATGGAA   541 GACACTAAAG GAAAAAATTA ACGACAAAGA CAGCACCAAC AGATGTCGTT GTTCCAGAGC   601 TGATGAGGGG TATCTCGAAG CACACGAAAC TTTTTCCTTC CTTCATTCAC GCACACTACT   661 CTCTAATGAG CAACGGTATA CGGCCTTCCT TCCAGTTACT TGAATTTGAA ATAAAAAAAA   721 GTTTGCTGTC TTGCTATCAA GTATAAATAG ACCTGCAATT ATTAATCTTT TGTTTCCTCG   781 TCATTGTTCT CGTTCCCTTT CTTCCTTGTT TCTTTTTCTG CACAATATTT CNNGCTATAC   841 CAAGCNTACA ATCNNCTATC TCATATACNG CGGNCGCATG GTGANCNANG GNNNANGANG   901 ATAACATGGN CNTCNTCAAN GANTTCATGC NCNTNNANNN GCANNTGGNN GGGCTNCNNG 961 AACNGNNCNN NNNTNCNANN NCGANGGCNN NNGNNNNNNN NNN //
Primer: pUC-CAPbs_up_F2 Region: T3 pro, insert 3' (CYC1 ter, MCS, mCherry 3') Sequence file: RDB19395_B1K1b.seq
>D08418D5_B1K1_1_pUC-CAPbs_up_F2_A02_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NCTTCCGGCT CNNATGTTGT    61 GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA   121 AGCTCGAAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGT ACCGGCCGCA AATTAAAGCC   181 TTCGAGCGTC CCAAAACCTT CTCAAGCAAG GTTTTCAGTA TAATGTTACA TGCGTACACG   241 CGTCTGTACA GAAAAAAAAG AAAAATTTGA AATATAAATA ACGTTCTTAA TACTAACATA   301 ACTATAAAAA AATAAATAGG GACCTAGACT TCAGGTTGTC TAACTCCTTC CTTTTCGGTT   361 AGAGCGGATG TGGGGGGAGG GCGTGAATGT AAGCGTGACA TAACTAATTA CATGACTCGA   421 GGTCGACGGT ATCGATAAGC TTGATATCGA ATTCCTGCAG CCCGGGGGAT CCACTAGTCT   481 TGTACAGCTC GTCCATGCCG CCGGTGGAGT GGCGGCCCTC GGCGCGTTCG TACTGTTCCA   541 CGATGGTGTA GTCCTCGTTG TGGGAGGTGA TGTCCAACTT GATGTTGACG TTGTAGGCGC   601 CGGGCAGCTG CACGGGCTTC TTGGCCTTGT AGGTGGTCTT GACCTCAGCG TCGTAGTGGC   661 CGCCGTCCTT CAGCTTCAGC CTCTGCTTGA TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT   721 ACATCCGCTC GGAGGAGGCC TCCCAGCCCA TGGTCTTCTT CTGCATNACG GGGCCGTCGG   781 AGGGGAAGTT GGTGCCGCGC ANCTTCACCT TGTAGANGAN CTCGCCNTCC TGCANGGNNG   841 AGTCCTGGGT CACGGTCACC ACNCCNCCNT CCTCNNANNN TCATCACGCG CTCCNNCTTG   901 AAGCCNTCNG GGAANGNCAG CTTCAAGNAN TCNGGGNNGT NNNNNGGGTG CTTCACGNAN   961 GCCTTGNANC CNTNNNTGAA CTGAGGGNNA GGNNGTCCCN NNNNNNNGGC AGGGGGNCNN  1021 CNNNGGNNNN NNCANNNNNN NNNCNGNNNN CCNCNNNNGG NNGNNNNNGC CNNNNNNNNN 1081 N //
Primer: URA3pro_F Region: URA3 pro, URA3 Sequence file: RDB19395_B1K1c.seq
>D08418D5_B1K1_1_URA3pro_F_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNATTCTTAA CCCANNCTGC    61 ACAGNAACAA AAACCTGCAG GAAACGAAGA TAAATCATGT CGAAAGCTAC ATATAAGGAA   121 CGTGCTGCTA CTCATCCTAG TCCTGTTGCT GCCAAGCTAT TTAATATCAT GCACGAAAAG   181 CAAACAAACT TGTGTGCTTC ATTGGATGTT CGTACCACCA AGGAATTACT GGAGTTAGTT   241 GAAGCATTAG GTCCCAAAAT TTGTTTACTA AAAACACATG TGGATATCTT GACTGATTTT   301 TCCATGGAGG GCACAGTTAA GCCGCTAAAG GCATTATCCG CCAAGTACAA TTTTTTACTC   361 TTCGAAGACA GAAAATTTGC TGACATTGGT AATACAGTCA AATTGCAGTA CTCTGCGGGT   421 GTATACAGAA TAGCAGAATG GGCAGACATT ACGAATGCAC ACGGTGTGGT GGGCCCAGGT   481 ATTGTTAGCG GTTTGAAGCA GGCGGCAGAA GAAGTAACAA AGGAACCTAG AGGCCTTTTG   541 ATGNNNNNAG AATTGTCATG CAAGGGCTCC CTATCTACTG GAGANNNNNN TAAGGGTACT   601 GTTGACATTG CGAAGAGCGA CAAAGATTTT GTTATCGGCT TTATTGCTCA AAGAGACATG   661 GGTGGAAGAG ATGAAGGTTA CGATTGGTTG ANTATGACAC CCGGTGTGGG TTTAGATGAC   721 AAGGGAGACG CNTTGGGTCA ACAGTATAGA ACCGTGGATG ATGTGGTCTC TACAGGATCT   781 GACNTTATTA TTGTTGNAAG ANGACTATTT GCNAAGGGAN NGGATGCTAN NGTANNNGGT   841 GAACGTNACN GAAAAAGCNN GCTGGGAAGC ATATTTGANA NNNNGCNNNC CAGCAAAACT   901 AAAAAAANTG TATTNNTNAG NNAANNGCAT GNTATNCTAA ACNNNACAAA NNTANNNNNT   961 NCNNTTTNNA NNTNATANNC NNNTNNTNNN NNNTNNGCNG NNNNNNAANN NNNCNNNNNN 1021 NNNN //
Primer: ADH1pro_F Region: insert mid (ADH1 pro, mCherry 5') Sequence file: RDB19395_B1K1d.seq
>D08418D5_B1K1_1_ADH1pro_F_E03_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN TTTGNNNNNN GTNNTTGTTC TCGTTCCCTT TCTTCCTTGT    61 TTCTTTTTCT GCACAATATT TCAAGCTATA CCAAGCATAC AATCAACTAT CTCATATACA   121 GCGGCCGCAT GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC   181 GCTTCAAGGT GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG   241 GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC   301 CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG   361 TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT   421 GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC   481 TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG   541 GCCCCGTAAT GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG   601 AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT   661 ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT   721 ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCNNGGAAC   781 AGTACGAACG CGCCGAGGGC CGCCACTCCA NCNGCGGCAT GGACGANCTG TNCAAGACTA   841 GTGGATCCCC CGGGCTGCAN GAANTCGATA TCAAGCTTAT CGATACCGNC NANCTCNAGT   901 CATGTAATTA GTTATGTCAN NCTNACNTNC NCGCCCNNCC CCCACATNCG CTCTNACCGA   961 AANNGAAGGA GTTAGANNAN NTGAAGNCTA NGNNCCNNTT ATTTTTTTAN ANNNNNGNTN 1021 GNNNNNNNAC GNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.08.12

GNP_filter3_RDBDEP_html_220812.pl