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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Target BFP gene expression plasmid (#RDB19359)

Expression vector of target BFP gene, a point mutant (T199C) of GFP for artificial enzymatic RNA editing system.

Clone info. Expression vector of target blue fluorescent protein (BFP) gene, a point mutant (T199C) of GFP for artificial enzymatic RNA editing system. Please refer to Bhakta, S. et al. Sci. Rep. 10 (1): 17304, 2020.
Comment Expression was confirmed by the depositor with microscopy.
Vector backbone pcDNA3 (plasmid)
Depositor|Developer Tsukahara, Toshifumi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Bhakta, S. et al., Sci. Rep. 10 (1): 17304, 2020)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Bhakta, S. et al., Sci. Rep. 10 (1): 17304, 2020)。

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RDB19359 Target BFP gene expression plasmid DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Target BFP gene expression plasmid was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19359).

Reference section:

Bhakta, S., Sakari, M., Tsukahara, T., RNA editing of BFP, a point mutant of GFP, using artificial APOBEC1 deaminase to restore the genetic code. Sci. Rep. 2020 Oct 14;10(1):17304 (2020). PMID 33057101. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19359_B1Jqp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,T7 pro,insert 5'
Sequence file: RDB19359_B1Jqa.seq check
>19359_19359_B1Jg_2_CMV-Forward_B03.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TGGGCGGTAG GCGNNNNNNG NGGGAGGTCT
   61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA
  121 ATACGACTCA CTATAGGGAG ACCCAAGCTT CCGGCGGTTC TGACATCCGG CGGTTCTGAC
  181 ATCCGGCGGT TCTGACATCC GGCGGTTCTG ACATCCGGCG GTTCTGACAT AAGCTTGGTA
  241 CCGAGCTCGG ATCCCACCAT GGACTACAAA GACGATGACG ATAAAGGGGG CGGTGGAGGT
  301 TACCCATACG ATGTTCCAGA TTACGCTGGT GGAGGTGGAG GTGAATTCAT GGTGAGCAAG
  361 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC
  421 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC
  481 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC
  541 CTGTCCCACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
  601 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC
  661 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC
  721 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC
  781 AACTTCNACA GCCACAACGT CTATATCATG GCCGACAAGC ANAAGAACGG CATCNAGGCG
  841 AANTTCAAGA TCCGCCACAA CATCGAGGAN GGCAGCGTGC AGCTCGCCNA NNNCTACNNN
  901 CAGAACACCC CCNTCGGCGA NNGNCCCNNG CTGNTGCCCG ACAACCACTA NCTGANCNNC
  961 CNNTNCGCCC TGANCAAGAN CCCANNGANN ANNGCGATNN CNTGGNCCTG CTGGNNTTCG
1021 TGNNCNNCNC CNGGNTNNCT CNCGGCANGN NNNNNCNNNN NNNNTNNNAN NNNNNNNNN
//
Primer: bGH_rev3
Sequence file: RDB19359_B1Jqb.seq check
>19359_19359_B1Jg_2_bGH_rev3_C03.ab1
    1 NNNNNNNNNN NNNGNNGNCT GGCAACTAGA AGGCACAGTC GAGGCTGATC AGCGAGCTCT
   61 AGCATTTAGG TGACACTATA GAATAGGGCC CTCTAGATGC ATGCTCGAGC GGCCGCCAGT
  121 GTGATGGATA TCTTACTTGT ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC
  181 GAACTCCAGC AGGACCATGT GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG
  241 GGTGCTCAGG TAGTGGTTGT CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG
  301 CTGGTAGTGG TCGGCGAGCT GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT
  361 CGCCTTGATG CCGTTCTTCT GCTTGTCGGC CATGATATAG ACGTTGTGGC TGTTGAAGTT
  421 GTACTCCAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC
  481 GATGCGGTTC ACCAGGGTGT CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC
  541 GTCGTCCTTG AAGAAGATGG TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA
  601 GAAGTCGTGC TGCTTCATGT GGTCGGGGTA GCGGCTGAAG CACTGCACGC CGTGGGACAG
  661 GGTGGTCACG AGGGTGGGCC AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG
  721 GGTCAGCTTG CCGTANGTGG CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGCC
  781 GTTTACGTCG CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC
  841 CTTGCTCACC ATGAATTCNC CTCCNCCTNC ACCAGCGTAA TCTGGAANAT CGNATGGGTA
  901 ACCNCCNCNG CCCCCTTTAT CGNCNTCGNC TTTGTANNCC ANGGNGGGNN NNGAGCTCNG
  961 TACCNNNCTT ANGTCANANN CNNNNNGNNG NCNNANNNNN CNNGNNNNNN NANNCNNNNN
1021 GNANNNNNNN NNN
//
Primer: SV40pro_ori_F
Sequence file: RDB19359_B1Jqc.seq check
>19359_19359_B1Jg_2_SV40pro_ori_F_D03.ab1
    1 NNNNNNNNNN CNNCTGCCTC TGAGCTATTC NNGAAGTAGT GAGGAGGCTT TTTTGGAGGC
   61 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG
  121 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC
  181 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT
  241 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG
  301 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG
  361 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA
  421 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA
  481 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC
  541 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC
  601 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG
  661 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG
  721 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT
  781 GTGGCGGANC GCTATCANGN CATAGCGTTG GCTACNCGTG ATATTGCTGA AGANCTTGGC
  841 GGCGAATGGG CTGANCGCTT CCTCGTGCTT TACGGTATCG CCNCTCCCGA TTCGCANCGC
  901 ATCGCCNTCT ATCGCCTTCN NGANNANTNC NNCTGANCNG GANTCTGGGG TTCNAAATGA
  961 NCGANCNAGC NNCNCCCNNN CNNGNCNNCN NNNNATTTCN ATNNCNCCNN NNNCNNNNNN
1021 NNNAANNNTN NGNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Bhakta, S., RNA editing of BFP, a point mutant of GFP, using artificial APOBEC1 deaminase to restore the genetic code. Sci. Rep. 2020 Oct 14;10(1):17304 (2020). PMID 33057101. [link to RRC of NBRP]