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pGL4_α-satellite-ZFAT box-4

Luciferase reporter vector of ZFAT box-4 in the alpha-satellite DNA.

Catalog number RDB19354
Resource name pGL4_α-satellite-ZFAT box-4
Clone info. PCR amplified reporter constructs containing a part of alpha-satellite DNA. The nucleotide sequence for the ZFAT box-4 in the reporter construct containing CENP-B box is GAATGTGC. See Fig.2A, 2B and Table S2 of Ishikura, S. et al. Nucleic Acids Res. 48 (19): 10848-10866, 2020.
Comment Expression was confirmed by the depositor with luciferase assay.
Vector backbone pGL4.14-E1bTATA (plasmid)
Size of vector backbone 5.8 kb
Selectable markers Ampicillin
Depositor|Developer Shirasawa, Senji | Ishikura, Shuhei |
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            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. (Ishikura, S. et al., Nucleic Acids Res. 48 (19): 10848-10866, 2020) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
            Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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            提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Ishikura, S. et al., Nucleic Acids Res. 48 (19): 10848-10866, 2020)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行うこと。
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            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB19354 pGL4_α-satellite-ZFAT box-4 Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Electronic file

            Original reference

            original Ishikura, S., ZFAT binds to centromeres to control noncoding RNA transcription through the KAT2B-H4K8ac-BRD4 axis. Nucleic Acids Res. 48 (19): 10848-10866 (2020). PMID 32997115.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            References

            Original, user report and related articles

            original Ishikura, S., ZFAT binds to centromeres to control noncoding RNA transcription through the KAT2B-H4K8ac-BRD4 axis. Nucleic Acids Res. 48 (19): 10848-10866 (2020). PMID 32997115.

            2022.05.16

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