Resource data sheet
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pFN21A_Halo_CK1delta_K38R (#RDB19315)

Expresion vector of human CK1 delta kinase-dead mutant (K38R) tagged with Halo at N-terminus.

Alternative name pFN21A_Halo_CK1δ_K38R
Clone info. Expresion vector of human casein kinase 1 delta (CK1 delta) kinase-dead mutant (K38R) tagged with Halo at N-terminus. See Fig.6 of Kinoshita-Kikuta, E. et al. Sci. Rep. 10 (1): 16273, 2020.
Comment Expression was confirmed by the depositor with SDS-PAGE, immunoblotting.
Vector backbone pFN21A HaloTag CMV Flexi (plasmid)
Size of vector backbone 5.1 kb
Selectable markers Ampicillin
Gene/insert name human CSNK1D cDNA
Depositor|Developer Kinoshita, Emiko |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kinoshita-Kikuta, E. et al., Sci. Rep. 10 (1): 16273, 2020)
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kinoshita-Kikuta, E. et al., Sci. Rep. 10 (1): 16273, 2020)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19315 pFN21A_Halo_CK1delta_K38R Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pFN21A_Halo_CK1delta_K38R was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19315).

Reference section:

Kinoshita-Kikuta, E., Utsumi, T., Miyazaki, A., Tokumoto, C., Doi, K., Harada, H., Kinoshita, E., Koike, T., Protein-N-myristoylation-dependent phosphorylation of serine 13 of tyrosine kinase Lyn by casein kinase 1γ at the Golgi during intracellular protein traffic. Sci. Rep. 10 (1): 16273 (2020). PMID 33004926. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Kinoshita-Kikuta, E., Protein-N-myristoylation-dependent phosphorylation of serine 13 of tyrosine kinase Lyn by casein kinase 1γ at the Golgi during intracellular protein traffic. Sci. Rep. 10 (1): 16273 (2020). PMID 33004926. [link to RRC of NBRP]