Resource data sheet
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pCDNA3.1_HCK_G2A_FLAG (#RDB19297)

Expresion vector of human HCK proto-oncogene non-N-myristoylated mutant (G2A) tagged with FLAG at C-terminus.

Alternative name pCDNA3.1_HCK_G2A_FLAG
Clone info. Expresion vector of human HCK proto-oncogene, Src family tyrosine kinase (HCK) non-N-myristoylated mutant (G2A) tagged with FLAG at C-terminus. See Fig.1 of Kinoshita-Kikuta, E. et al. Sci. Rep. 10 (1): 16273, 2020.
Comment Expression was confirmed by the depositor with SDS-PAGE, immunoblotting.
Vector backbone pCDNA3.1(-) (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name human HCK cDNA
Depositor|Developer Kinoshita, Emiko |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kinoshita-Kikuta, E. et al., Sci. Rep. 10 (1): 16273, 2020)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kinoshita-Kikuta, E. et al., Sci. Rep. 10 (1): 16273, 2020)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19297 pCDNA3.1_HCK_G2A_FLAG Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

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How to cite this biological resource

Materials & Methods section:

The pCDNA3.1_HCK_G2A_FLAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19297).

Reference section:

Kinoshita-Kikuta, E., Utsumi, T., Miyazaki, A., Tokumoto, C., Doi, K., Harada, H., Kinoshita, E., Koike, T., Protein-N-myristoylation-dependent phosphorylation of serine 13 of tyrosine kinase Lyn by casein kinase 1γ at the Golgi during intracellular protein traffic. Sci. Rep. 10 (1): 16273 (2020). PMID 33004926. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

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References

Original, user report and related articles

original Kinoshita-Kikuta, E., Protein-N-myristoylation-dependent phosphorylation of serine 13 of tyrosine kinase Lyn by casein kinase 1γ at the Golgi during intracellular protein traffic. Sci. Rep. 10 (1): 16273 (2020). PMID 33004926. [link to RRC of NBRP]