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pCMV-SEC24

Expression vector of human SEC24-P2A-moxGFP under the control of CMV promoter.

Catalog number RDB19285
Resource name pCMV-SEC24
Clone info. Expression vector of human SEC24 homolog B, COPII coat complex component (SEC24)-P2A-moxGFP under the control of CMV promoter. See Fig.1B and Fig.5A of Mori, Y. et al. Sci. Rep. 10 (1): 4798, 2020. This clone is used for the protein expression limit estimation method. Refer to the experimental procedure.
Expression was confirmed by the depositor with flowcytometry.
Vector backbone pTOW40836 (plasmid)
Selectable markers Ampicillin (E. coli), URA3, leu2-89 (S. cerevisiae).
Gene/insert name human SEC24B cDNA
Depositor|Developer Moriya, Hisao |
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External Database
human SEC24B

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Mori, Y. et al. Sci. Rep. 10 (1): 4798, 2020).
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Schedule A (GFP-A) [Link]
          Remarks
          Please use Schedule A (GFP-A) additionally.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mori, Y. et al. Sci. Rep. 10 (1): 4798, 2020)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          Schedule A(GFP-A, 英語版のみ) [Link]
          備考
          Schedule A(GFP-A, 英語版のみ)が追加で必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB19285 pCMV-SEC24 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
          Ordering Information [in Japanese] [in English]

          References and tips

          Electronic file

          Original reference

          original Mori, Y., Development of an experimental method of systematically estimating protein expression limits in HEK293 cells. Sci. Rep. 10 (1): 4798 (2020). PMID 32179769.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Mori, Y., Development of an experimental method of systematically estimating protein expression limits in HEK293 cells. Sci. Rep. 10 (1): 4798 (2020). PMID 32179769.

          2022.01.12

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