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pCMV-SEAP delta SS

Expression vector of SEAP mutant-P2A-moxGFP under the control of CMV promoter.

Catalog number RDB19284
Resource name pCMV-SEAP delta SS
Clone info. Expression vector of secreted alkaline phosphatase (SEAP) mutant-P2A-moxGFP under the control of CMV promoter. SEAP is a nonfluorescent target protein, SEAP mutants lack a signal sequence (SS) from SEAP. See Fig.1B and Fig.4A of Mori, Y. et al. Sci. Rep. 10 (1): 4798, 2020. This clone is used for the protein expression limit estimation method. Refer to the experimental procedure.
Expression was confirmed by the depositor with flowcytometry.
Vector backbone pTOW40836 (plasmid)
Selectable markers Ampicillin (E. coli), URA3, leu2-89 (S. cerevisiae).
Gene/insert name secreted alkaline phosphatase
Depositor|Developer Moriya, Hisao |
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External Database
secreted alkaline phosphatase

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Mori, Y. et al. Sci. Rep. 10 (1): 4798, 2020).
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Schedule A (GFP-A) [Link]
            Remarks
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mori, Y. et al. Sci. Rep. 10 (1): 4798, 2020)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            Schedule A(GFP-A, 英語版のみ) [Link]
            備考
            Schedule A(GFP-A, 英語版のみ)が追加で必要です。

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            RDB19284 pCMV-SEAP delta SS Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
            Ordering Information [in Japanese] [in English]

            References and tips

            Electronic file

            Original reference

            original Mori, Y., Development of an experimental method of systematically estimating protein expression limits in HEK293 cells. Sci. Rep. 10 (1): 4798 (2020). PMID 32179769.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            References

            Original, user report and related articles

            original Mori, Y., Development of an experimental method of systematically estimating protein expression limits in HEK293 cells. Sci. Rep. 10 (1): 4798 (2020). PMID 32179769.

            2022.01.12

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