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pCMV-RFP*

Expression vector of mCherry-noncleaving P2A mutant-moxGFP under the control of CMV promoter.

Catalog number RDB19281
Resource name pCMV-RFP*
Clone info. Expression vector of mCherry-noncleaving P2A mutant-moxGFP under the control of CMV promoter. See Fig.1B and Fig.3D of Mori, Y. et al. Sci. Rep. 10 (1): 4798, 2020. This clone is used for the protein expression limit estimation method. Refer to the experimental procedure.
Expression was confirmed by the depositor with western blotting.
Vector backbone pTOW40836 (plasmid)
Selectable markers Ampicillin (E. coli), URA3, leu2-89 (S. cerevisiae).
Depositor|Developer Moriya, Hisao |
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            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Mori, Y. et al. Sci. Rep. 10 (1): 4798, 2020).
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            Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose)[Link]
            Remarks
            The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mori, Y. et al. Sci. Rep. 10 (1): 4798, 2020)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            専用MTA (Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用)[Link]
            備考
            本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。専用MTA(Takara Bio USA, Inc. FPs, 英語版のみ、非営利機関による非営利学術研究用)をご参照ください。

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            RDB19281 pCMV-RFP* Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
            Ordering Information [in Japanese] [in English]

            References and tips

            Electronic file

            Original reference

            original Mori, Y., Development of an experimental method of systematically estimating protein expression limits in HEK293 cells. Sci. Rep. 10 (1): 4798 (2020). PMID 32179769.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            References

            Original, user report and related articles

            original Mori, Y., Development of an experimental method of systematically estimating protein expression limits in HEK293 cells. Sci. Rep. 10 (1): 4798 (2020). PMID 32179769.

            2022.01.12

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