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pCMV-RFP

Expression vector of mCherry-P2A-moxGFP under the control of CMV promoter.

Catalog number RDB19280
Resource name pCMV-RFP
Clone info. Expression vector of mCherry-P2A-moxGFP under the control of CMV promoter. See Fig.1B and Fig.3A of Mori, Y. et al. Sci. Rep. 10 (1): 4798, 2020. This clone is used for the protein expression limit estimation method. Refer to the experimental procedure.
Comment Expression was confirmed by the depositor with fluorescent microscope, western blotting.
Vector backbone pTOW40836 (plasmid)
Selectable markers Ampicillin (E. coli), URA3, leu2-89 (S. cerevisiae).
Depositor|Developer Moriya, Hisao |
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            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Mori, Y. et al. Sci. Rep. 10 (1): 4798, 2020).
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            This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please contact us to request the Exclusive MTA.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mori, Y. et al. Sci. Rep. 10 (1): 4798, 2020)。
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            RDB19280 pCMV-RFP Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            How to cite this biological resource

            Materials & Methods section:

            The pCMV-RFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19280).

            Reference section:

            Mori, Y., Yoshida, Y., Satoh, A., Moriya, H., Development of an experimental method of systematically estimating protein expression limits in HEK293 cells. Sci. Rep. 10 (1): 4798 (2020). PMID 32179769. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Mori, Y., Development of an experimental method of systematically estimating protein expression limits in HEK293 cells. Sci. Rep. 10 (1): 4798 (2020). PMID 32179769. [link to RRC of NBRP]

            2023.06.15

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