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pCMV-MTS-GFP

Expression vector of human mitochondrial protein Cox8-moxGFP under the control of CMV promoter.

Catalog number RDB19278
Resource name pCMV-MTS-GFP
Clone info. Expression vector of human mitochondrial protein Cox8-moxGFP (MTS-moxGFP) under the control of CMV promoter. See Fig.1B and 2A of Mori, Y. et al. Sci. Rep. 10 (1): 4798, 2020. This clone is used for the protein expression limit estimation method. Refer to the experimental procedure.
Comment Expression was confirmed by the depositor with flowcytometry and western blotting.
Vector backbone pTOW40836 (plasmid)
Selectable markers Ampicillin (E. coli), URA3, leu2-89 (S. cerevisiae).
Depositor|Developer Moriya, Hisao |
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            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Mori, Y. et al. Sci. Rep. 10 (1): 4798, 2020).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mori, Y. et al. Sci. Rep. 10 (1): 4798, 2020)。
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            RDB19278 pCMV-MTS-GFP Under QC test. Please contact us. DNA solution

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            References and tips

            Original reference

            original Mori, Y., Development of an experimental method of systematically estimating protein expression limits in HEK293 cells. Sci. Rep. 10 (1): 4798 (2020). PMID 32179769.

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Mori, Y., Development of an experimental method of systematically estimating protein expression limits in HEK293 cells. Sci. Rep. 10 (1): 4798 (2020). PMID 32179769.

            2023.04.06

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