Plasmid vector used for gene manipulation in fungi, Repression type, Hygromycin B-resistant selective marker. Gateway(R) cloning technology vector.
Clone info. | Plasmid vector used for gene manipulation in fungi, Repression type, Hygromycin B-resistant selective marker, Introduced into fungal cells by Agrobacterium tumefaciens-mediated transformation, Easy insertion of the objective gene by Gateway technology. Structure of pFungiway5 vector, see Fig.1 of Nishikawa, R. et al. Ann. Microbiol. 66 (2), 825-832, 2016. |
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Comment | Recommendation from Gene Engineering Division, Riken BRC. Use 10-20 µg/mL of hygromycin with chloramphenicol for culture of transformant to reduce the possibility to obtain plasmids with deletion between homologous sequences within the plasmid. |
Vector backbone | pCAMBIA2200 (plasmid) |
Selectable markers | Chloramphenicol (bacterial selection), Kanamycin (plant selection) |
Growth remarks | ccdB survival |
Reference of insert sequence | LC029922 (DDBJ accession number) |
Depositor|Developer | Shimosaka, Makoto | |
Sequence (full) | RDB19270hts01.seq Assembled from experimentally sequenced data. |
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Publication | Nishikawa, R., Annals of Microbiology 66: 825-832 (2016). [link to RRC of NBRP] |
Test sheet | RDB19270_B1J4p1-1.pdf |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nishikawa, R. et al., Ann. Microbiol. 66 (2), 825-832, 2016) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nishikawa, R. et al., Ann. Microbiol. 66 (2), 825-832, 2016)。非営利機関の利用者に限定、学術研究の利用に限定。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB19270 | pFungiway5 | DNA solution |
Materials & Methods section:
The pFungiway5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19270). |
Reference section:
Nishikawa, R., Yoshida, M., Noda, T., Okuhara, T., Taguchi, G., Inatomi, S., Shimosaka, M., pFungiway: a series of plasmid vectors used for gene manipulation in fungi. Annals of Microbiology 66: 825-832 (2016). [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Nishikawa, R., pFungiway: a series of plasmid vectors used for gene manipulation in fungi. Annals of Microbiology 66: 825-832 (2016). [link to RRC of NBRP] |
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