Resource data sheet
DNA Bank Top
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

pFungiway3 (#RDB19268)

Plasmid vector used for gene manipulation in fungi, Expression type, G418-resistant selective marker. Gateway(R) cloning technology vector.

Clone info. Plasmid vector used for gene manipulation in fungi, Expression type, G418-resistant selective marker, Introduced into fungal cells by Agrobacterium tumefaciens-mediated transformation, Easy insertion of the objective gene by Gateway technology. Structure of pFungiway3 vector, see Fig.1 of Nishikawa, R. et al. Ann. Microbiol. 66 (2), 825-832, 2016.
Vector backbone pCAMBIA2200 (plasmid)
Selectable markers Chloramphenicol (bacterial selection), Kanamycin (plant selection)
Growth remarks ccdB survival
Reference of insert sequence LC029920 (DDBJ accession number)
Depositor|Developer Shimosaka, Makoto |
 
Sequence (full) RDB19268hts01.seq checkAssembled from experimentally sequenced data.
Publication Nishikawa, R., Annals of Microbiology 66: 825-832 (2016). [link to RRC of NBRP]
Test sheet RDB19268_B1J4p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nishikawa, R. et al., Ann. Microbiol. 66 (2), 825-832, 2016) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nishikawa, R. et al., Ann. Microbiol. 66 (2), 825-832, 2016)。非営利機関の利用者に限定、学術研究の利用に限定。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19268 pFungiway3 DNA solution

How to cite this biological resource

Materials & Methods section:

The pFungiway3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19268).

Reference section:

Nishikawa, R., Yoshida, M., Noda, T., Okuhara, T., Taguchi, G., Inatomi, S., Shimosaka, M., pFungiway: a series of plasmid vectors used for gene manipulation in fungi. Annals of Microbiology 66: 825-832 (2016). [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nishikawa, R., pFungiway: a series of plasmid vectors used for gene manipulation in fungi. Annals of Microbiology 66: 825-832 (2016). [link to RRC of NBRP]