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pFungiway1 (#RDB19266)

Plasmid vector used for gene manipulation in fungi, Expresion type, Hygromycin B-resistant selective marker. Gateway(R) cloning technology vector.

Clone info. Plasmid vector used for gene manipulation in fungi, Expresion type, Hygromycin B-resistant selective marker, Introduced into fungal cells by Agrobacterium tumefaciens-mediated transformation, Easy insertion of the objective gene by Gateway technology. Structure of pFungiway1 vector, see Fig.1 of Nishikawa, R. et al. Ann. Microbiol. 66 (2), 825-832, 2016.
Comment Recommendation from Gene Engineering Division, Riken BRC.
Use 10-20 µg/mL of hygromycin with chloramphenicol for culture of transformant to reduce the possibility to obtain plasmids with deletion between homologous sequences within the plasmid.
Vector backbone pCAMBIA2200 (plasmid)
Selectable markers Chloramphenicol (bacterial selection), Kanamycin (plant selection)
Growth remarks ccdB survival
Reference of insert sequence LC029918 (DDBJ accession number)
Depositor|Developer Shimosaka, Makoto |
 
Sequence (full) RDB19266hts01.seq checkAssembled from experimentally sequenced data.
Publication Nishikawa, R., Annals of Microbiology 66: 825-832 (2016). [link to RRC of NBRP]
Test sheet RDB19266_B1J4p1-1.pdf 
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nishikawa, R. et al., Ann. Microbiol. 66 (2), 825-832, 2016) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nishikawa, R. et al., Ann. Microbiol. 66 (2), 825-832, 2016)。非営利機関の利用者に限定、学術研究の利用に限定。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19266 pFungiway1 DNA solution


How to cite this biological resource

Materials & Methods section:

The pFungiway1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19266).

Reference section:

Nishikawa, R., Yoshida, M., Noda, T., Okuhara, T., Taguchi, G., Inatomi, S., Shimosaka, M., pFungiway: a series of plasmid vectors used for gene manipulation in fungi. Annals of Microbiology 66: 825-832 (2016). [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Nishikawa, R., pFungiway: a series of plasmid vectors used for gene manipulation in fungi. Annals of Microbiology 66: 825-832 (2016). [link to RRC of NBRP]