RDB19260 - px330-mC/4933436l01Rik

px330-mC/4933436l01Rik_Down

Expression vector of sgRNA targeting downstream of mouse 4933436I01Rik and hSpCas9-mouse Cdt1.

Catalog number	RDB19260
Resource name	px330-mC/4933436l01Rik_Down
Clone info.	Expression vector of sgRNA targeting downstream of mouse 4933436I01Rik and hSpCas9-mouse Cdt1 fusion protein, cloned into the px330-mC (RDB14406).
Comment	Activity was confirmed by the depositor. In vitro CRISPR-cutting pCAG-EGxxFP cloned sgRNA target sequence and checking EGFP signal.
Vector backbone	px330-mC (plasmid)
Size of vector backbone	8.8 kb
Selectable markers	Ampicillin
Gene/insert name	mouse 4933436I01Rik cDNA
Depositor\|Developer	Mizuno, Seiya \|

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External Database
mouse 4933436I01Rik

Reference sequence

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Morimoto, K. et al., Sci. Rep., 10 (1): 9060, 2020).

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Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Morimoto, K. et al., Sci. Rep., 10 (1): 9060, 2020).
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Remarks	The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Morimoto, K. et al., Sci. Rep., 10 (1): 9060, 2020)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 専用MTA（CRISPR/Cas9内包遺伝子材料専用非営利学術目的）をお使いください [Word]
備考	本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB19260	px330-mC/4933436l01Rik_Down	Under QC test. Please contact us.	DNA solution

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

Electronic file

Original reference

original	Morimoto, K., Reverse genetics reveals single gene of every candidate on Hybrid sterility, X Chromosome QTL 2 (Hstx2) are dispensable for spermatogenesis. Sci. Rep. 10 (1): 9060 (2020). PMID 32493902.

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

Original, user report and related articles

original	Morimoto, K., Reverse genetics reveals single gene of every candidate on Hybrid sterility, X Chromosome QTL 2 (Hstx2) are dispensable for spermatogenesis. Sci. Rep. 10 (1): 9060 (2020). PMID 32493902.

2022.05.16

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