Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

px330-mC/Gm1140-Gm14692_Mid

Expression vector of sgRNA targeting interspace between Gm1140 and Gm14692 and hSpCas9-mouse Cdt1.

Catalog number RDB19257
Resource name px330-mC/Gm1140-Gm14692_Mid
Clone info. Expression vector of sgRNA targeting interspace between Gm1140 and Gm14692 and hSpCas9-mouse Cdt1 fusion protein, cloned into the px330-mC (RDB14406).
Comment Activity was confirmed by the depositor. In vitro CRISPR-cutting pCAG-EGxxFP cloned sgRNA target sequence and checking EGFP signal.
Vector backbone px330-mC (plasmid)
Size of vector backbone 8.8 kb
Selectable markers Ampicillin
Gene/insert name mouse Gm1140 cDNA mouse Gm14692 cDNA
Depositor|Developer Mizuno, Seiya |
Other clones in our bank

External Database
mouse Gm1140 mouse Gm14692

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Morimoto, K. et al., Sci. Rep., 10 (1): 9060, 2020).
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
        Remarks
        The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
        提供案内 (日本国内) [open/close]

        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Morimoto, K. et al., Sci. Rep., 10 (1): 9060, 2020)。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        専用MTA(CRISPR/Cas9内包遺伝子材料専用 非営利学術目的)をお使いください [Word]
        備考
        本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

        Catalog # Resource name Availability Shipping form Fee (non-profit org.)
        RDB19257 px330-mC/Gm1140-Gm14692_Mid Under QC test. Please contact us. DNA solution

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        References and tips

        Electronic file

        Original reference

        original Morimoto, K., Reverse genetics reveals single gene of every candidate on Hybrid sterility, X Chromosome QTL 2 (Hstx2) are dispensable for spermatogenesis. Sci. Rep. 10 (1): 9060 (2020). PMID 32493902.

        Further references such as user reports and related articles (go to bottom)

        Featured content


        Sequence information

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


        References

        Original, user report and related articles

        original Morimoto, K., Reverse genetics reveals single gene of every candidate on Hybrid sterility, X Chromosome QTL 2 (Hstx2) are dispensable for spermatogenesis. Sci. Rep. 10 (1): 9060 (2020). PMID 32493902.

        2022.05.16

        GNP_filter3_RDBDEP_html_220516.pl