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EGFP3

Expression vector of tandemly oligomerized EGFP for diffusion analysis of proteins, multiple oligomeric EGFPn (n=3) with molecular weights of 90 kD.

Catalog number RDB19233
Resource name EGFP3
Clone info. Expression vector of tandemly oligomerized EGFP for diffusion analysis of proteins, multiple oligomeric EGFPn (n=3) with molecular weights of 90 kD, which can be used as molecular rulers for quantification of protein mobility in the nucleus. See Pack, C.G. et al., Biophys. J., 91 (10): 3921-3936, 2006.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pEGFP-C1 (plasmid)
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Kinjo, Masataka | Kitamura, Akira |
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External Database
Aequorea victoria GFP

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Pack, C.G. et al., Biophys. J., 91 (10): 3921-3936, 2006).
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Pack, C.G. et al., Biophys. J., 91 (10): 3921-3936, 2006)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB19233 EGFP3 Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Electronic file

            Original reference

            original Pack, C., Microenvironment and effect of energy depletion in the nucleus analyzed by mobility of multiple oligomeric EGFPs. Biophys. J. 91 (10): 3921-3936 (2006). PMID 16950841.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            References

            Original, user report and related articles

            original Pack, C., Microenvironment and effect of energy depletion in the nucleus analyzed by mobility of multiple oligomeric EGFPs. Biophys. J. 91 (10): 3921-3936 (2006). PMID 16950841.

            2022.07.13

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