Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA5-FRT-TO-EGFP-Mo DP1 (#RDB19223)

Expression vector of mouse DP1/Reep5 tagged with EGFP.

Clone info. Expression vector of mouse receptor accessory protein 5 (DP1/Reep5) tagged with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with immunofluorescence microscopy.
Vector backbone pcDNA5-FRT-TO (plasmid)
Size of vector backbone 5.1 kb
Selectable markers Ampicillin (E. coli), Hygromycin (mammalian cell)
Gene/insert name mouse Reep5 cDNA
Depositor|Developer Nukina, Nobuyuki | Yamanaka, Tomoyuki |
 

Distribution information

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yamanaka, T. et al., Sci. Rep., 10 (1): 7610, 2020).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yamanaka, T. et al., Sci. Rep., 10 (1): 7610, 2020)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19223 pcDNA5-FRT-TO-EGFP-Mo DP1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA5-FRT-TO-EGFP-Mo DP1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19223).

Reference section:

Yamanaka, T., Nishiyama, R., Shimogori, T., Nukina, N., Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation. Sci. Rep. 10 (1): 7610 (2020). PMID 32376919. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19223_B1Gjp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, tetO2, EGFP 5'
Sequence file: RDB19223_B1Gja.seq check
1
Primer: EGFP-C2 (Pr0402)
Region: EGFP 3', insert 5'
Sequence file: RDB19223_B1Gjb.seq check
1
Primer: BGH_rev2 (Pr0606)
Region: insert 3'
Sequence file: RDB19223_B1Gjc.seq check
1
Primer: BGHpA_F (Pr0373)
Region: bGHpA, FRT, HygR
Sequence file: RDB19223_B1Gjd.seq check
1

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yamanaka, T., Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation. Sci. Rep. 10 (1): 7610 (2020). PMID 32376919. [link to RRC of NBRP]