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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA5-FRT-TO-EGFP-Mo Climp63

Expression vector of mouse Climp63/Ckap4 tagged with EGFP.

Catalog number RDB19222
Resource name pcDNA5-FRT-TO-EGFP-Mo Climp63
Clone info. Expression vector of mouse cytoskeleton-associated protein 4 (Climp63/Ckap4) tagged with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with immunofluorescence microscopy.
Vector backbone pcDNA5-FRT-TO (plasmid)
Size of vector backbone 5.1 kb
Selectable markers Ampicillin (E. coli), Hygromycin (mammalian cell)
Gene/insert name mouse Ckap4 cDNA
Depositor|Developer Nukina, Nobuyuki | Yamanaka, Tomoyuki |
Other clones in our bank
External Database
mouse Ckap4
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yamanaka, T. et al., Sci. Rep., 10 (1): 7610, 2020).
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yamanaka, T. et al., Sci. Rep., 10 (1): 7610, 2020)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB19222 pcDNA5-FRT-TO-EGFP-Mo Climp63 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Yamanaka, T., Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation. Sci. Rep. 10 (1): 7610 (2020). PMID 32376919.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19222_B1Gjp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro, tetO2, EGFP 5'
          Sequence file: RDB19222_B1Gja.seq check
          1
          Primer: EGFP-C2
          Region: EGFP 3', insert 5'
          Sequence file: RDB19222_B1Gjb.seq check
          1
          Primer: BGH_rev2
          Region: insert 3'
          Sequence file: RDB19222_B1Gjc.seq check
          1
          Primer: BGHpA_F
          Region: bGHpolyA, FRT, HygR
          Sequence file: RDB19222_B1Gjd.seq check
          1

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Yamanaka, T., Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation. Sci. Rep. 10 (1): 7610 (2020). PMID 32376919.

          2022.07.13

          GNP_filter3_RDBDEP_html_220713.pl